CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Post-translational: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
生物活性
Immobilized Recombinant Human CD86 Protein (rp217153) at 1.0 μg/mL can bind CD86 Mouse mAb (Ab095464) with the EC50 of 28.0 ng/mL.
39.1-61.4 kDa, under reducing conditions; 40.0-57.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human B7-2/CD86 Protein (rp217153) - ELISA Immobilized Recombinant Human B7-2/CD86 Protein (rp217153) at 1.0 μg/mL can bind CD86 Mouse mAb (Ab095464) with the EC₅₀ of 28.0 ng/mL.
Recombinant Human B7-2/CD86 Protein (rp217153) - SDS-PAGE 3 μg/lane of Recombinant Human B7-2/CD86 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.1-61.4 kDa under reducing conditions and 40.0-57.4 kDa under non-reducing conditions.