无动物源, 无载体, Fc标签, ≥90%(SDS-PAGE), expressed in HEK293; 见COA
生化机理
Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain. Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor. Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain (gamma c) to form the functional high affinity IL-7 receptor complex. The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor. IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors. It is expressed early in B cell development, prior to the appearance of surface IgM. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. In human, by contrast, it is required for T cell but not for B cell development. IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha. IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin. TSLP indirectly regulates T cell development by modulating dendritic cell activation. Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion. The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R.
Post-translational: N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. Ubiquitinated by MARCHF8; leading to lysosomal degradation.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-7R alpha/CD127 Protein (rp220385) - SDS-PAGE
3 μg/lane of Recombinant Human IL-7R alpha/CD127 Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing the band at 62.4-78.0 kDa.
Recombinant Human IL-7R alpha/CD127 Protein (rp220385) - SDS-PAGE
3 μg/lane of Recombinant Human IL-7R alpha/CD127 Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing the band at 62.4-78.0 kDa.
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