Recombinant Human HuR/ELAVL1 Protein

货号: rp220390
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥85%(SDS-PAGE) expressed in E. coli; 见COA
Accession #
Q15717
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC50 of 8.13 ng/mL.
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规格
库存
价格
数量
10μg
rp220390-10μg
现货 Stock Image
¥999.90
50μg
rp220390-50μg
现货 Stock Image
¥2,999.90
100μg
rp220390-100μg
现货 Stock Image
¥4,799.90
1mg
rp220390-1mg
期货 Stock Image
¥23,999.90
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为什么选择此级别

ActiBioPure™,His标签,无载体,生物活性 级 提供 ≥85%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human HuR/ELAVL1 Protein
规格或纯度
无载体, 生物活性, ActiBioPure™, His标签, ≥85%(SDS-PAGE), expressed in E. coli; 见COA
生化机理
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010). Post-translational: Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462). Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
生物活性
Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC50 of 8.13 ng/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
2-326 aa
序列
MHHHHHHSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
36.9 kDa
SDS-PAGE
33.0 kDa, under reducing conditions; 33.3 & 66.6 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 500mM NaCl, 5% Glycerol, 100mM Imidazole,​ pH 8.0
浓度
expressed in E. coli; 见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human HuR/ELAVL1 Protein (rp220390) - ELISA Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC₅₀ of 8.13 ng/mL.
Recombinant Human HuR/ELAVL1 Protein (rp220390) - SDS-PAGE 
3 μg/lane of Recombinant Human HuR/ELAVL1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 33.0 kDa under reducing conditions and 33.3 & 66.6 kDa under non-reducing conditions. HuR/ELAVL1 can form homodimers (Uniprot: Q15717; PubMed:17632515, 20219472). The two bands of HuR/ELAVL1 monomer correspond to its two conformations under non-reducing conditions (PMID: 21723170).

技术文档

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ26F0939086 分析证书 rp220390
ZJ26F0939085 分析证书 rp220390
ZJ26F0939084 分析证书 rp220390
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