级别和纯度: 无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥85%(SDS-PAGE)expressed in E. coli; 见COA
Accession #
Q15717
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC50 of 8.13 ng/mL.
无载体, 生物活性, ActiBioPure™, His标签, ≥85%(SDS-PAGE), expressed in E. coli; 见COA
生化机理
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969).
Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity).
Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947).
Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:8626503).
Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503).
With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity).
Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
Post-translational: Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462). Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
生物活性
Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC50 of 8.13 ng/mL.
Recombinant Human HuR/ELAVL1 Protein (rp220390) - ELISA
Immobilized Recombinant Human HuR/ELAVL1 Protein (rp220390) at 1.0 μg/mL can bind Recombinant HuR/ELAVL1 Antibody (Ab109133) with the EC₅₀ of 8.13 ng/mL.
Recombinant Human HuR/ELAVL1 Protein (rp220390) - SDS-PAGE
3 μg/lane of Recombinant Human HuR/ELAVL1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 33.0 kDa under reducing conditions and 33.3 & 66.6 kDa under non-reducing conditions.
HuR/ELAVL1 can form homodimers (Uniprot: Q15717; PubMed:17632515, 20219472). The two bands of HuR/ELAVL1 monomer correspond to its two conformations under non-reducing conditions (PMID: 21723170).