无动物源,无载体,Fc标签,≥85%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Interleukin 35 (IL-35) is a member of the IL-12 family of heterodimeric cytokines. Unlike other IL-12 family cytokines which stimulate the immune response, the predominant function of IL-35 is as an immunosuppressant. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-35 are the IL-12 p35 subunit and the EBI3 subunit, respectively. The IL-12 p35 subunit of IL-35 is synthesized as a 219 amino acid (aa) precursor protein with a 22 aa signal sequence and a 197 aa mature region. The EBI3 subunit of IL-35 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-35 share 58% and 62% sequence homology in their IL-12 p35 and EBI3 subunits, respectively. IL-35 binds to the homodimeric receptors, IL-12 R beta 2 and gp130, as well as to the IL-12 R beta 2-gp130 receptor heterodimer. The expression pattern of IL-35 is thought to differ between mouse and humans. In mouse regulatory T cells, both subunits of IL-35 are constitutively expressed and the mature IL-35 is secreted. In humans, IL-12 p35 is the only subunit constitutively expressed in regulatory T cells. Immune activation can induce EBI3 expression and IL-35 secretion in human effector T cells. IL-35 is also expressed and secreted in human placental trophoblasts. In both human and mouse IL-35 has been shown to suppress effector T cell proliferation, inhibit Th17 cell development, and promote the conversion of T cells and B cells into regulatory T and B cells, respectively. IL-35 is thought to be involved in infectious tolerance and inflammatory cytokine-mediated autoimmune disorders. Serum levels of IL-35 are associated with acute graft-versus-host disease following hematopoietic stem cell transplantation. IL-35 also functions as a regulator of tumor growth.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-35 Protein (rp222542) - SDS-PAGE
3 μg/lane of Recombinant Human IL-35 Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing a band at 87.7-99.2 kDa.
危险品化学品经营许可证（带存储）