级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
Q9GZV9
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 35.4 ng/mL in the presence of Recombinant Mouse Klotho beta.
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Fibroblast growth factor 23 (FGF‑23) is a 30‑32 kDa member of the FGF family, within a subfamily that also includes FGF‑19 and FGF‑21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta ‑trefoil structure. FGF‑19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma‑stabilizing intramolecular folds. Mature human FGF‑23 contains an atypical (very low affinity) heparin binding site (aa 134‑162), a proteolytic cleavage site (Arg179‑Ser180), and multiple O‑linked glycosylation sites with Thr178 being of particular importance. O‑linked glycosylation at Thr178 blocks the cleavage of FGF‑23, thereby preventing loss of FGF‑23 activity. Mature human FGF‑23 shows 72% aa identity to mouse FGF‑23 and is active on mouse cells. FGF‑23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF‑23 activity. FGF‑23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels. FGF‑23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by down‑regulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate.
Post-translational: Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably.
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 35.4 ng/mL in the presence of Recombinant Mouse Klotho beta.
27.2-34.0 kDa; under reducing conditions; 26.3-32.7 kDa; under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 2mM DTT, 1mM EDTA, 10% Glycerol, pH 7.4
浓度
expressed in HEK293; 见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃ 存储6个月；收货后建议分装，避免反复冻融。
图片
Recombinant Human FGF23 Protein (rp222570) - Protein Bioactivity
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 35.4 ng/mL in the presence of Recombinant Mouse Klotho beta.
Recombinant Human FGF23 Protein (rp222570) - SDS-PAGE
3 μg/lane of Recombinant Human FGF23 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.2-34.0 kDa under reducing conditions and 26.3-32.7 kDa under non-reducing conditions.
危险品化学品经营许可证（带存储）