acid stable subunit of the 140 K IGF complex | binding protein 29 | binding protein 53 | growth hormone-dependent binding protein | IBP-3 | IBP3BP-53 | IGF-binding protein 3 | IGFBP3 | IGFBP-3 | insulin-like growth factor binding protein 3 | insulin-like
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,PBS Only,≥90%(SDS-PAGE),See COA
生化机理
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:10874028, PubMed:19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed:20353938).
Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed:9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed:15371331).
In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed:10874028).
Post-translational: Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2; resulting in decreased nuclear localization (PubMed:19556345).
生物活性
Measured by its ability to inhibit the biological activity of IGF-II on MCF‑7 human breast cancer cells. The ED50 for this effect is 0.13 µg/mL.
38.5-50.5 kDa, under reducing conditions; 38.9-51.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
See COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human IGFBP-3 Protein (rp222573) - Protein Bioactivity
Measured by its ability to inhibit the biological activity of IGF-II on MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 0.13 µg/mL.
Recombinant Human IGFBP-3 Protein (rp222573) - SDS-PAGE
3 μg/lane of Recombinant Human IGFBP-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.5-50.5 kDa under reducing conditions and 38.9-51.5 kDa non-reducing conditions.