级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P24593
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.
Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18930415, PubMed:7683690).
Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells (By similarity).
Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling (By similarity).
Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs (PubMed:20345844, PubMed:26103640).
Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling (PubMed:36949068).
Post-translational: Cleaved by C1S in extracellular space.
生物活性
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.
30.1-35.5 kDa, under reducing conditions; 29.2-34.9 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IGFBP5 Protein (rp222575) - Protein Bioactivity
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.
Recombinant Human IGFBP5 Protein (rp222575) - SDS-PAGE
3 μg/lane of Recombinant Human IGFBP5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 30.1-35.5 kDa under reducing conditions and 29.2-34.9 kDa under non-reducing conditions.
ZJ26F0939469
危险品化学品经营许可证（带存储）