级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P24592
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 0.62 µg/mL in the presence of 14 ng/mL rhIGF-II.
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Activates the MAPK signaling pathway and induces cell migration (PubMed:24003225).
Post-translational: O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.
生物活性
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 0.62 µg/mL in the presence of 14 ng/mL rhIGF-II.
26.2-35.0 kDa, under reducing conditions; 26.3-35.0 & 53.4-61.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IGFBP6 Protein (rp222576) - Protein Bioactivity
Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 0.62 µg/mL in the presence of 14 ng/mL rhIGF-II.
Recombinant Human IGFBP6 Protein (rp222576) - SDS-PAGE
3 μg/lane of Recombinant Human IGFBP6 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 26.2-35.0 kDa under reducing conditions and 26.3-35.0 & 53.4-61.0 kDa under non-reducing conditions.
Human IGFBP6 Protein can form the dimers through covalent interaction via disulfide bonds under non-reducing conditions (Uniprot: P24592).
危险品化学品经营许可证（带存储）