级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
O00292
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human LEFTY2 Protein (rp222591) at 1.0 μg/mL can bind Recombinant LEFTY1/LEFTY2 Antibody (Ab112968) with the EC50 of 6.7 ng/mL.
无动物源,无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.
Post-translational: The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
生物活性
Immobilized Recombinant Human LEFTY2 Protein (rp222591) at 1.0 μg/mL can bind Recombinant LEFTY1/LEFTY2 Antibody (Ab112968) with the EC50 of 6.7 ng/mL.
43.4 kDa, under reducing conditions; 39.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human LEFTY2 Protein (rp222591) - ELISA
Immobilized Recombinant Human LEFTY2 Protein (rp222591) at 1.0 μg/mL can bind Recombinant LEFTY1/LEFTY2 Antibody (Ab112968) with the EC₅₀ of 6.7 ng/mL.
Recombinant Human LEFTY2 Protein (rp222591) - SDS-PAGE
3 μg/lane of Recombinant Human LEFTY2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 43.4 kDa under reducing conditions and 39.4 kDa under non-reducing conditions.