级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)expressed in E. coli; 见COA
无载体,His标签,≥95%(SDS-PAGE),expressed in E. coli; 见COA
生化机理
E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling (PubMed:25266658, PubMed:33964137). Plays a critical role in the regulation of nuclear lamina (PubMed:33964137).
Post-translational: Auto-ubiquitinated; leads to proteasomal degradation.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human RNF220 Protein (rp225011) - SDS-PAGE
3 μg/lane of Recombinant Human RNF220 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 41.5 & 85.7 kDa under reducing conditions.
The observation of protein as both dimers and monomers under reducing (R) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot: Q5VTB9).
危险品化学品经营许可证（带存储）