Immobilized Recombinant Human IL-1 beta/IL-1F2 Protein (rp226952) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 88.6 ng/mL.
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in humans. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
Post-translational: Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.(Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1.
生物活性
Immobilized Recombinant Human IL-1 beta/IL-1F2 Protein (rp226952) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 88.6 ng/mL.
16.7 kDa, under reducing conditions; 16.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-1 beta/IL-1F2 Protein (rp226952) - ELISA Immobilized Recombinant Human IL-1 beta/IL-1F2 Protein (rp226952) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 88.6 ng/mL.
Recombinant Human IL-1 beta/IL-1F2 Protein (rp226952) - SDS-PAGE 3 μg/lane of Recombinant Human IL-1 beta/IL-1F2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.7 kDa.
ZJ25F1028620
危险品化学品经营许可证（带存储）