Recombinant Human IL-18 Protein

Name: Recombinant Human IL-18 Protein
SKU: rp228973
Availability: InStock

货号: rp228973

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? His标签 ? ≥95%(SDS-PAGE) 见COA

Accession #

Q14116

蛋白标签

N-His

表达系统

E. coli

内毒素水平

<1.0 EU/μg

生物活性

Immobilized Recombinant Human IL-18 Protein (rp228973) at 2.0 μg/mL can bind GSK 1070806 (anti-IL-18) (Ab177855) with the EC50 of 39.4 ng/mL.

规格

库存

价格

数量

10μg

rp228973-10μg

期货

¥619.90

50μg

rp228973-50μg

期货

¥1,999.90

100μg

rp228973-100μg

期货

¥3,129.90

1mg

rp228973-1mg

期货

¥16,699.90

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规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器

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为什么选择此级别

ActiBioPure™,His标签,无动物源,无载体,生物活性级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称

Recombinant Human IL-18 Protein

别名

重组人IL-18蛋白 | 重组人白介素-18蛋白

英文别名

规格或纯度

无动物源, 无载体, 生物活性, ActiBioPure™, His标签, ≥95%(SDS-PAGE), 见COA

生化机理

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. Post-translational: The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18.

生物活性

Immobilized Recombinant Human IL-18 Protein (rp228973) at 2.0 μg/mL can bind GSK 1070806 (anti-IL-18) (Ab177855) with the EC50 of 39.4 ng/mL.

表达系统

E. coli

种属

人(Human)

氨基酸

37-193 aa

序列

MGSSHHHHHHSSGLVPRGSHMYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

蛋白标签

N-His

无载体

Yes

无动物源

Yes

来源

重组表达

预测分子量

20.5 kDa

SDS-PAGE

17.8 kDa, under reducing conditions; 17.8 kDa, under non-reducing conditions.

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4.

浓度

见COA

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

-20℃保存 12个月；复溶后建议分装，避免反复冻融。

图片

ELISA: Recombinant Human IL-18 Protein (rp228973)

Recombinant Human IL-18 Protein (rp228973) - ELISA

Immobilized Recombinant Human IL-18 Protein (rp228973) at 2.0 μg/mL can bind GSK 1070806 (anti-IL-18) (Ab177855) with the EC50 of 39.4 ng/mL.

SDS-PAGE: Recombinant Human IL-18 Protein (rp228973)

Recombinant Human IL-18 Protein (rp228973) - SDS-PAGE

3 μg/lane of Recombinant Human IL-18 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.8 kDa under reducing conditions and 17.8 kDa under non-reducing conditions.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 His Tag 级别指南 →

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Recombinant Human IL-18 Protein

储存与运输

质量文档

文献证明

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

需要帮助选择级别？

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