Immobilized Recombinant Human Sonic Hedgehog N-Terminus Protein (rp228981) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 17.3 ng/mL.
HHG1 | HHG-1 | HLP3 | HPE3 | MCOPCB5 | MCOPCB5sonic hedgehog (Drosophila) homolog | Shh | ShhNC | SMMCI | SMMCIsonic hedgehog homolog (Drosophila) | sonic hedgehog homolog | sonic hedgehog protein | Sonic Hedgehog | TPT | TPTPS
规格或纯度
无动物源,PBS Only,≥95%(SDS-PAGE),见COA
生化机理
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
Post-translational: Sonic hedgehog protein
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity (By similarity).Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (ShhN) (By similarity).Cholesterylation is required for the sonic hedgehog protein N-product targeting to lipid rafts and multimerization (PubMed:24522195, PubMed:26875496).ShhN is the active species in both local and long-range signaling, whereas the C-product (ShhC) is degraded in the endoplasmic reticulum (By similarity).
Sonic hedgehog protein N-product
N-palmitoylation by HHAT of ShhN is required for sonic hedgehog protein N-product multimerization and full activity (By similarity). It is a prerequisite for the membrane-proximal positioning and the subsequent shedding of this N-terminal peptide (PubMed:24522195).
Sonic hedgehog protein N-product
The lipidated N- and C-terminal peptides of ShhNp can be cleaved (shedding) (PubMed:24522195). The N-terminal palmitoylated peptide is cleaved at the Cardin-Weintraub (CW) motif site (PubMed:24522195). The cleavage reduced the interactions with heparan sulfate. The cleavage is enhanced by SCUBE2 (PubMed:23118222, PubMed:24522195).
生物活性
Immobilized Recombinant Human Sonic Hedgehog N-Terminus Protein (rp228981) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 17.3 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human Sonic Hedgehog Protein (rp228981) - ELISA
Immobilized Recombinant Human Sonic Hedgehog Protein (rp228981) at 1.0 μg/mL can bind Sonic Hedgehog Mouse mAb (Ab128686) with the EC50 of 17.3 ng/mL.
Recombinant Human Sonic Hedgehog Protein (rp228981) - SDS-PAGE
3 μg/lane of Recombinant Human Sonic Hedgehog Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 17.6-22.7 & 40.1-44.1 kDa under reducing conditions and 17.8-23.5 & 36.6-45.6 & 58.8-66.3 kDa under non-reducing conditions.
Sonic Hedgehog can form Multimer. Multiple bands correspond to multiple conformations, which caused by incomplete autocleavage in animal-component-free medium (Uniprot: Q15465).
ZJ25F1029172
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