级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P29460,Q9NPF7
蛋白标签
C-12His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human IL-23R Protein ( rp147522) at 2.0 μg/mL can bind Recombinant Human IL23 Protein (rp229094) with the EC50 of 67.55 ng/mL. Immobilized Recombinant Human IL23 Protein (rp229094) at 1.0 μg/mL can bind Guselkumab (anti-IL-23) (Ab175717) with the EC50 of 57.38 ng/mL.
无动物源,无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
Post-translational: Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
生物活性
Immobilized Recombinant Human IL-23R Protein ( rp147522) at 2.0 μg/mL can bind Recombinant Human IL23 Protein (rp229094) with the EC50 of 67.55 ng/mL. Immobilized Recombinant Human IL23 Protein (rp229094) at 1.0 μg/mL can bind Guselkumab (anti-IL-23) (Ab175717) with the EC50 of 57.38 ng/mL.
61.4 kDa, under reducing conditions; 54.0- 62.0 &124.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-23 Protein (rp229094) - Binding Activity
Immobilized Recombinant Human IL-23R Protein ( rp147522) at 2.0 μg/mL can bind Recombinant Human IL-23 Protein (rp229094) with the EC50 of 67.55 ng/mL.
Recombinant Human IL-23 Protein (rp229094) - ELISA
Immobilized Recombinant Human IL-23 Protein (rp229094) at 1.0 μg/mL can bind Guselkumab (anti-IL-23) (Ab175717) with the EC₅₀ of 57.38 ng/mL.
Recombinant Human IL-23 Protein (rp229094) - SDS-PAGE
3 μg/lane of Recombinant Human IL-23 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 61.4 kDa under reducing conditions and 54.0-62.0 & 124.0 kDa under non-reducing conditions.
危险品化学品经营许可证（带存储）