级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
Q16552,Q96PD4
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human IL-17A&IL-17F Protein (rp229111) at 1.0 μg/mL can bind Bimekizumab (anti-IL17A&IL-17F) (Ab182793) with the EC50 of 8.3 ng/mL.
无动物源,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:24120361).
Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed:17911633, PubMed:18684971, PubMed:19825828, PubMed:21350122, PubMed:24120361, PubMed:8676080).
Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity).
In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines (By similarity).
In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells (By similarity).
Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance (By similarity).
Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation (By similarity).
Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection (PubMed:21350122).
Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis (By similarity).
As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release (By similarity).
In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity).
Involved in antiviral host defense through various mechanisms (By similarity).
Enhances immunity against West Nile virus by promoting T cell cytotoxicity (By similarity).
May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung (By similarity).
Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense (By similarity
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:21350122).
IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed:11574464, PubMed:11591732, PubMed:11591768, PubMed:17911633, PubMed:18684971, PubMed:21350122, PubMed:28827714).
IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation (By similarity).
As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity).
Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity).
IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway (PubMed:32187518).
Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi. Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression (By similarity).
Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes (By similarity)..
Post-translational: N-glycosylated. Found both in glycosylated and nonglycosylated forms.
生物活性
Immobilized Recombinant Human IL-17A&IL-17F Protein (rp229111) at 1.0 μg/mL can bind Bimekizumab (anti-IL17A&IL-17F) (Ab182793) with the EC50 of 8.3 ng/mL.
32.3-40.5 kDa, under reducing conditions; 30.0-39.1 & 54.5-67.6 & 95.7-110.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-17A & IL-17F Protein (rp229111) - ELISA
Immobilized Recombinant Human IL-17A & IL-17F Protein (rp229111) at 1.0 μg/mL can bind Bimekizumab (anti-IL17A & IL-17F) (Ab182793) with the EC50 of 8.3 ng/mL.
Recombinant Human IL-17A & IL-17F Protein (rp229111) - SDS-PAGE
3 μg/lane of Recombinant Human IL-17A & IL-17F Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 32.3-40.5 kDa under reducing conditions and 30.0-39.1 & 54.5-67.6 & 95.7-110.4 kDa under non-reducing conditions.
There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID: Q16552-1 & Q96PD4-1).
危险品化学品经营许可证（带存储）