Luciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening.
生物活性
Testing in progress
表达系统
E. coli
种属
Photinus pyralis
氨基酸
2-550 aa (T214N, A222C, Y255F, S276T, H332N, E354N)
58.4 kDa, under reducing conditions; 58.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 1mM DTT, 10% Glycerol, pH 8.0
浓度
expressed in E. coli; 见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃(6个月);收货后建议分装,避免反复冻融。
图片
Recombinant Photinus Pyralis Luciferase Protein (rp231176) - SDS-PAGE
3 μg/lane of Recombinant Photinus Pyralis Luciferase Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 58.4 kDa.