Recombinant Human Insulin Protein

货号: rp231842
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥95%(SDS-PAGE) 见COA
Accession #
P01308
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.17 μg/mL.
★
规格
库存
价格
数量
10μg
rp231842-10μg
现货 Stock Image
¥799.90
50μg
rp231842-50μg
现货 Stock Image
¥2,299.90
100μg
rp231842-100μg
现货 Stock Image
¥3,669.90
1mg
rp231842-1mg
现货 Stock Image
¥16,699.90
请输入您要添加的产品数量
🧪
为什么选择此级别

ActiBioPure™,His标签,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human Insulin Protein
别名
重组人Insulin蛋白 | 重组人胰岛素蛋白
英文别名
IDDM2 | ILPR | insulin | IRDN | MODY10 | Proinsulin
规格或纯度
无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥95%(SDS-PAGE), 见COA
生化机理
Proinsulin is synthesized as a single chain, 110 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and an 86 aa proinsulin propeptide. Following removal of the signal peptide, the proinsulin peptide undergoes further proteolysis to generate mature insulin, a 51 aa disulfide-linked dimer that consists of a 30 aa B chain (aa 25 - 54) bound to a 21 aa A chain (aa 90 - 110). The 34 aa intervening peptide (aa 55 - 89) that connects the B and A chains is termed the C-peptide. Human proinsulin shares 84% and 80% aa sequence identity with rat and bovine proinsulin, respectively. Most of the sequence variation between species occurs in the region of the C-peptide. This peptide generates a structural conformation that allows for the correct formation of the intrachain disulphide bonds. Insulin is a molecule that facilitates the cellular uptake of glucose. This is accomplished by regulating the appearance of membrane glucose transporters. Low insulin levels or lack of insulin are associated with type 2 and type 1 diabetes mellitus, respectively. These conditions are associated with an increased risk for microvascular complications such as retinopathy, nephropathy, and peripheral neuropathy. Proinsulin also circulates, but its physiologic role is less well understood. It does possess about 25% of the activity of mature insulin, but it would seem unlikely to be a natural substitute for insulin. In type 2 diabetes, an elevated proinsulin to insulin ratio in the circulation is a well-known abnormality. Perhaps this abnormality represents either compromised proteolytic processing or a general inability to process increased levels of insulin precursor. In any event, proinsulin will stimulate amylin secretion by beta-cells, and amyloid formation in pancreatic islets that promotes decreased beta cell function. Studies also suggest that fasting serum proinsulin may be a better predictor of future type 2 diabetes than fasting insulin levels in obese children.
生物活性
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.17 μg/mL.
表达系统
E. coli
种属
人(Human)
氨基酸
25-53 & 90-110 aa
序列
MHHHHHHFVNQHLCGSHLVEALYLVCGERGFFYTPKAAKGIVEQCCTSICSLYQLENYCN
蛋白标签
N-His
无载体
Yes
无动物源
No
来源
重组表达
预测分子量
6.9 kDa
SDS-PAGE
6.0 & 13.2 kDa, under reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 20% Glycerol, pH 7.4
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片

Recombinant Human Insulin Protein (rp231842) - Bioactivity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.17 μg/mL.

Recombinant Human Insulin Protein (rp231842) - SDS-PAGE
3 μg/lane of Recombinant Human Insulin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 6.0 & 13.2 kDa under reducing conditions.
Insulin contains disulfide bonds, which may contribute to the formation of aggregates (Uniprot: P01308).

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到4个结果

批号(Lot Number) 证书类型 货号
ZJ25F1028546 分析证书 rp231842
ZJ25F1028547 分析证书 rp231842
ZJ25F1028548 分析证书 rp231842
ZJ25F1028549 分析证书 rp231842
溶液计算器