6-phosphogluconate dehydrogenase (PGD) is the second dehydrogenase in the pentose phosphate pathway. It catalyzes the oxidative decarboxylation of
6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH. Together with glucose-6-phosphate dehydrogenase (G6PD), these enzymes ensure the reducing environment of the cell cytoplasm. However, the activities of both enzymes are strongly inhibited by NADPH. It is not quite clear how the enzymes are regulated to maintain the level of reducing power inside cells. Deficiency of PGD is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of PGD and 6-phosphogluconolactonase suggest a synergism of the two enzymopathies.
50.0 kDa, under reducing conditions; 48.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM HEPES, 200mM NaCl, 2mM TCEP, pH 7.5
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human 6-Phosphogluconate Dehydrogenase/PGD Protein (rp232637) - SDS-PAGE 3 μg/lane of Recombinant Human 6-Phosphogluconate Dehydrogenase/PGD Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 50.0 kDa under reducing conditions and 48.7 kDa under non-reducing conditions.
ZJ25F1028368
危险品化学品经营许可证（带存储）