级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in E. coli; 见COA
Accession #
Q9Y243
蛋白标签
N-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human AKT3 Protein (rp301924) at 1.0 μg/mL can bind Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) with the EC50 of 1.373 ng/mL.
DKFZP434N0250 | PKB gamma | PKBgamma/Akt3 | PKBgamma1 | Protein kinase Akt-3 | Protein kinase B gamma | RAC-gamma serine/threonine protein kinase | RAC-gamma serine/threonine-protein kinase | RAC-PK-gamma | similar to rat serine-threonine protein kinase |
规格或纯度
无载体, His标签, ≥90%(SDS-PAGE), expressed in E. coli; 见COA
生化机理
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Post-translational: Phosphorylation on Thr-305 and Ser-472 is required for full activity (PubMed:12162751, PubMed:9512493).
Phosphorylation of the activation loop at Thr-305 by PDPK1/PDK1 is a prerequisite for full activation (PubMed:9512493).
Phosphorylation at Ser-472 by mTORC2 in response to growth factors plays a key role in AKT1 activation by facilitating subsequent phosphorylation of the activation loop by PDPK1/PDK1 (By similarity).
生物活性
Immobilized Recombinant Human AKT3 Protein (rp301924) at 1.0 μg/mL can bind Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) with the EC50 of 1.373 ng/mL.
Immobilized Recombinant Human AKT3 Protein (rp301924) at 1.0 μg/mL can bind Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) with the EC50 of 1.373 ng/mL.
Recombinant Human AKT3 Protein (rp301924) - SDS-PAGE
2 μg/lane of Recombinant Human AKT3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56.5 kDa.
ZJ26F0737942
危险品化学品经营许可证（带存储）