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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁提供重组蛋白A/G与LumiDye™ AF染料的偶联物（表1）。
表1. 蛋白A/G偶联物的光谱特性
| Cat.No. | Conjugate | λmax (nm) | Em (nm |
| rp303272 | HRP | NA | NA |
| rp303273 | FITC | 498 | 517 |
| rp303275 | AF488 | 490 | 525 |
| rp303276 | AF555 | 553 | 568 |
| rp303277 | AF594 | 590 | 618 |
| rp303278 | AF647 | 650 | 668 |
| rp302416 | Cy3 | 554 | 566 |
阿拉丁重组蛋白A/G是一种基因工程蛋白，包含5个蛋白A的IgG结合区和2个蛋白G的IgG结合区。该重组A/G-Cys蛋白已去除了细胞壁结合区、细胞膜结合区和白蛋白结合区，以确保最大程度的特异性IgG结合。该重组蛋白A/G是制备用于纯化多克隆或单克隆IgG抗体的亲和凝胶的理想选择。蛋白A/G能结合多种人、小鼠和大鼠的IgG亚类（例如：人IgG1, IgG2, IgG3, IgG4；小鼠IgG2a, IgG2b, IgG3；大鼠IgG2a, IgG2c）。它还能结合牛、山羊、绵羊、马、兔、豚鼠、猪、狗和猫的总IgG。
我们检测了不同IgG亚型与重组蛋白A/G的结合数据，如图所示（图1）。
图1. 蛋白A/G对选定物种的亲和力
重组蛋白A/G 与HRP共价偶联，并经过分子筛纯化去除大部分未反应蛋白和HRP，得到高质量的偶联物。在蛋白质印迹法（WB）、酶联免疫吸附试验（ELISA）、免疫组化（IHC），免疫沉淀（IP） 及其他免疫检测方案中，用于探针标记与抗体检测，尤其适用于多种不同物种宿主的IgG 类抗体。
Aladdin offers recombinant protein A/G conjugated to our LumiDye™ AF Dyes (Table 1).
Table 1. Spectral characteristics of protein A/g conjugates.
| Cat.No. | Conjugate | λmax (nm) | Em (nm |
| rp303272 | HRP | NA | NA |
| rp303273 | FITC | 498 | 517 |
| rp303275 | AF488 | 490 | 525 |
| rp303276 | AF555 | 553 | 568 |
| rp303277 | AF594 | 590 | 618 |
| rp303278 | AF647 | 650 | 668 |
| rp302416 | Cy3 | 554 | 566 |
Aladdin recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant A/G-Cys to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for making affinity gel to purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.
We have detected the binding data of different IgG subtypes with recombinant protein A/G, as shown in the Graph1.
Graph 1. Protein A/G affinity to selected species.
Recombinant Protein A/G is covalently conjugated to HRP, followed by size‑exclusion chromatography to remove most unreacted proteins and free HRP, yielding a high‑quality conjugate. It is used for probing and antibody detection in Western blotting (WB), enzyme‑linked immunosorbent assay (ELISA), immunohistochemistry (IHC), Immunoprecipitation(IP) and other immunoassay protocols, and is especially suitable for IgG antibodies from a wide range of host species.
1/5000-1/100000
1/2000-1/20000
1:2000-1:20000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434798 |
分析证书 | rp303272 |
| ZJ26F0434799 |
分析证书 | rp303272 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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