级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。≥95%(SDS-PAGE)expressed in E. col; 见COA
Accession #
O95150
蛋白标签
No tag
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
TF-1 cells were co-stimulated with 5.0 μg/mL CHX (C112766) and 200.0 ng/mL Recombinant Human TL1A/TNFSF15 Protein (rp170417) for 24 hours. Cells were then stained with Annexin V-FITC/PI Apoptosis Detection Kit (A1372286) and analyzed by FACS.
无载体,ActiBioPure™,高性能,≥95%(SDS-PAGE),expressed in E. col; 见COA
生化机理
TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Human TL1A is a 251 aa protein consisting of a 35 aa cytoplasmic domain, a 24 aa transmembrane region and a 192 aa C-terminal extracellular domain. It is a longer variant of the previously cloned TL1 (also known as VEGI) that is possibly a cloning artifact. TL1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. DR3 is expressed primarily on activated T cells. Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways, activation of the transcription factor NF-kappa-B or activation of caspases and apoptosis. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A. Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response.
生物活性
TF-1 cells were co-stimulated with 5.0 μg/mL CHX (C112766) and 200.0 ng/mL Recombinant Human TL1A/TNFSF15 Protein (rp170417) for 24 hours. Cells were then stained with Annexin V-FITC/PI Apoptosis Detection Kit (A1372286) and analyzed by FACS.
19.8 kDa, under reducing conditions; 18.4 & 20.7 & 32.5 & 40.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in E. col; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融
图片
Recombinant Human TL1A/TNFSF15 Protein (rp310063) - Protein Bioactivity
TF-1 cells were co-stimulated with 5.0 μg/mL CHX (C112766) and 200.0 ng/mL Recombinant Human TL1A/TNFSF15 Protein (rp310063) for 24 hours. Cells were then stained with Annexin V-FITC/PI Apoptosis Detection Kit (A1372286) and analyzed by FACS.
Recombinant Human TL1A/TNFSF15 Protein (rp310063) - SDS-PAGE
3 μg/lane of Recombinant Human TL1A/TNFSF15 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 19.8 kDa under reducing conditions and 18.4 & 20.7 & 32.5 & 40.4 kDa under non-reducing conditions.
Homologous trimers linked by disulfide bonds (PMID: 17935696, PMID: 21300286) .
ZJ26F0333355
危险品化学品经营许可证（带存储）