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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Recombinant Human Wnt Surrogate Protein 可激活与市面上绝大多数重组 Wnt3a 蛋白、Wnt 条件培养基相近的 Wnt 信号通路,且所需蛋白用量更少,操作更简便。
本产品经基因工程改造,溶解性得到提升,攻克了 Wnt3a 应用中的一大难题,可降低模型体系中激活 Wnt 信号所需的蛋白使用浓度。 该蛋白由 DKK 的 LRP 结合结构域,与 Frizzled 受体选择性高亲和力结合模块连接而成。
靶点信息
Wnt3a 属于 WNT 基因家族,该家族包含一系列结构相关基因,编码分泌型信号蛋白。这类蛋白参与肿瘤发生以及多项发育生物学过程,包括胚胎发育过程中细胞命运调控与体轴模式建成。 WNT3a 为本家族成员。在畸胎瘤细胞系 NT2 中的表达研究表明,该基因可能参与维 A 酸诱导 NT2 细胞神经元分化的早期过程。该基因与同家族另一成员 WNT3,共同定位于 17 号染色体 q21 区域。
Recombinant Human Wnt Surrogate Protein activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle.
This product was engineered with improved solubility, overcoming a major obstacle of working with Wnt3a and reducing the concentrations required to activate Wnt signaling in your model system. Recombinant Human Wnt Surrogate Protein is comprised of the LRP-binding domain of DKK linked to a selective, high affinity binder of Frizzled receptors.
Target Information
Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
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