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土壤亚硝酸还原酶是反硝化作用中的关键酶之一，参与亚硝酸盐至NO的还原反应，它的活性反映了生物降解过程中氮素的转化效率，为氮素转化规律的研究提供一定的依据。 测定原理：亚硝酸还原酶可将NO₂⁻还原为NO，使样品中参与重氮化反应生成紫红色化合物的NO₂⁻减少，即540nm处吸光值的变化可反应土壤中亚硝酸还原酶的活性。
Soil Nitrite Reductase is one of the key enzymes in the denitrification process, catalyzing the reduction of nitrite to nitric oxide (NO). Its activity reflects the transformation efficiency of nitrogen during biodegradation and provides a basis for studying the patterns of nitrogen conversion. Assay Principle: Nitrite reductase reduces nitrite (NO₂⁻) to NO, thereby decreasing the amount of NO₂⁻ available to participate in the diazotization reaction that forms a purplish-red compound. Consequently, the change in absorbance at 540 nm reflects the activity of soil nitrite reductase.
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