蓝蛋白 (SHC) 是一种源于对虾的高可溶性血蓝蛋白，可替代钥孔帽贝血蓝蛋白 (KLH)，作为载体蛋白与多肽、核酸、药物或毒素等低分子量分子偶联，使其具备高免疫原性。经测试，SHC 具有与 KLH 相当的免疫原性，而其溶解度 ( 尤其是在与半抗原偶联后 ) 则显著高于 KLH，从而使免疫原制备方案具有更大的灵活性。
产品形式：血蓝蛋白以 PBS 溶解的冻干形式提供。使用时，可加入超纯水重悬。
产品特点
高免疫原性：可完美替代 KLH；
高得率偶联：偶联效率与 KLH 相同；
高溶解度：溶解度显著高于 KLH，兼容不常见或溶解性很差的半抗原；
高纯度：纯度大于 98%；
性能卓越：已经过数百例抗体制备验证；
高性价比：价格远低于 KLH。
使用方法
与半抗原偶联
1. 使用适量超纯水将 SHC (SMCC 活化 ) 冻干粉复溶为 10 mg/mL 的 SHC 溶液 ；
2. 将 20 mg 含有巯基的半抗原溶解于 5 mL 偶联缓冲液 (83 mM sodium phosphate, 0.1 M EDTA, 0.9 M NaCl, 0.1 mM TCEP, pH 7.2) 中；
注意：0.1 mM TCEP 不是必需的，它的主要作用是将半抗原的二硫键还原成巯基。
3. 立即将半抗原溶液与活化后的 SHC 按照质量比 1 : 0.6 混合，室温反应 2 h 或 4℃过夜反应；
注意：该偶联比例为推荐比例。不同半抗原大小和结构的差异会影响偶联效率，为确保更有效的偶联， 需根据具体情况，对半抗原和 SHC 偶联比例进行多次预实验验证。
4. 使用分子筛层析除去 EDTA。
注意事项
1. 本产品定量精确，请在原装管内按照所需浓度直接溶解使用；请勿对本产品进行分装，否则会因产品 性状 / 静电等原因造成较大损失；
2. SHC 预测消光系数ε1% 为 10，如需要测定 SHC 溶液浓度请调整消光系数；
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
4. 本产品仅限科研使用。
Shrimp Hemocyanin (SHC) is a highly soluble hemocyanin derived from shrimp. It can be used as an alternative to Keyhole Limpet Hemocyanin (KLH) as a carrier protein for conjugation with low-molecular-weight molecules such as peptides, nucleic acids, drugs or toxins to render them highly immunogenic. Tests have shown that SHC exhibits immunogenicity comparable to KLH, while its solubility (especially after conjugation with haptens) is significantly higher than that of KLH, allowing greater flexibility in immunogen preparation protocols.Product Format: Lyophilized powder dissolved in PBS. Reconstitute with ultrapure water before use.
Product Features
High Immunogenicity: Perfect replacement for KLH;
High Conjugation Yield: Conjugation efficiency identical to KLH;
High Solubility: Significantly higher solubility than KLH, compatible with uncommon or poorly soluble haptens;
High Purity: Purity > 98%;
Excellent Performance: Validated in hundreds of antibody preparation cases;
Cost-Effective: Price much lower than KLH.
Usage
Conjugation with Hapten
1. Reconstitute lyophilized SHC (SMCC-activated) with an appropriate volume of ultrapure water to a concentration of 10 mg/mL;
2. Dissolve 20 mg of thiol-containing hapten in 5 mL of conjugation buffer (83 mM sodium phosphate, 0.1 M EDTA, 0.9 M NaCl, 0.1 mM TCEP, pH 7.2);
Note: 0.1 mM TCEP is not essential. Its main function is to reduce disulfide bonds in the hapten to thiol groups.
3. Immediately mix the hapten solution with activated SHC at a mass ratio of 1:0.6. React for 2 h at room temperature or overnight at 4 °C;
Note: This conjugation ratio is for reference only. The size and structure of different haptens may affect conjugation efficiency. To ensure efficient conjugation, it is necessary to optimize the hapten-to-SHC ratio via several preliminary experiments according to specific conditions.
3. Remove EDTA by size-exclusion chromatography.
Notes
1. This product is accurately quantified. Please dissolve it directly in the original vial at the desired concentration. DO NOT aliquot the product, as significant loss may occur due to product characteristics / static electricity.
2. The predicted extinction coefficient ε1% of SHC is 10. Adjust the extinction coefficient when determining the concentration of SHC solution.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. For research use only.
ZJ26F0434004
危险品化学品经营许可证（带存储）