在组织切片过程中，一些组织内含有骨质或钙化灶时，含钙的组织不宜直接用石蜡包埋切片。这是因为钙和石蜡之间的密度不同，较难切出完整的切片。对含钙组织做好固定之后，再进行脱钙或二者同时进行。在脱钙过程中，酸类物质打破了组织中蛋白质分子间的横链，并释放可以同水分子结合的亲水基团，引起组织膨胀。组织脱钙后，要立即将组织中的酸中和掉，使组织迅速脱酸和中性化，以保证切片质量和染色效果。硫酸钠水溶液 (5%) 是较常用的骨组织脱钙后处理液，过夜处理。
操作步骤(仅供参考)：
1、骨组织脱钙时，取材不易过厚，一般大约5mm。
2、组织脱钙后用蒸馏水洗清洗3次，每次20min。
3、组织转移至20~30 倍体积的硫酸钠水溶液 (5% )中，处理24h 。
4、用蒸馏水冲洗数次。
注意事项：
1、适当加温能加快处理速度，一般不应超过37～40℃。
2、不建议采用水洗处理法，易引起组织膨胀。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、本产品仅用于科研，严禁他用。
附录：
乙醇处理法：70% 的乙醇处理，每 1h 更换一次，重复 2 次。
During tissue sectioning, when tissues contain bone or calcified foci, calcium-containing tissues are not suitable for direct paraffin embedding and sectioning. This is due to the density difference between calcium and paraffin, making it difficult to obtain intact sections. For calcium-containing tissues, fixation is preferably performed first, followed by decalcification, or the two processes can be carried out simultaneously. During decalcification, acidic substances break the cross-links between protein molecules in the tissue and release hydrophilic groups that can bind to water molecules, resulting in tissue swelling. After tissue decalcification, the acid in the tissue must be neutralized immediately to rapidly deacidify and neutralize the tissue, ensuring section quality and staining efficacy. Sodium Sulfate Aqueous Solution (5%) is a commonly used post-treatment solution for decalcified bone tissue, with overnight treatment.
Protocol (for reference only):
1. During bone tissue decalcification, the specimen thickness should not be excessive, generally approximately 5 mm.
2. Wash the decalcified tissue with distilled water 3 times, 20 minutes each time.
3. Transfer the tissue to a 5% aqueous sodium sulfate solution with a volume 20–30 times that of the tissue, and treat for 24 h.
4. Rinse the tissue with distilled water several times.
Precautions:
1. Appropriate heating can accelerate the treatment process, and the temperature should generally not exceed 37–40 ℃.
2. Water washing alone is not recommended, as it easily causes tissue swelling.
3. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
4. This product is for research use only; strictly prohibited for other purposes.
Appendix:
Ethanol treatment method: Treat with 70% ethanol, replace every 1 h, and repeat twice.
ZJ26F0434339
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