计算溶液所需的质量、体积或浓度。
土壤 N-乙酰-β-D-葡萄糖苷酶(S-NAG)广泛分布于各种组织中,溶酶体中的一种酸性水解酶,由土壤微生物分泌。S-NAG活性变化与机体某些病理状态密切相关。本试剂盒提供了一种简单、方便、快速的土壤 N-乙酰-β-D-葡萄糖苷酶活性检测方法。其原理是 S-NAG 分解 N-β-乙酰氨基葡萄糖苷生成对硝基苯酚,后者在400 nm 有最大吸收峰,通过测定 400 nm 下吸光度的变化来计算 NAG 活性。
Soil N-acetyl-β-D-glucosaminidase (S-NAG) is widely distributed in various tissues and is an acid hydrolase found in lysosomes, secreted by soil microorganisms. Changes in S-NAG activity are closely associated with certain pathological states in organisms. This kit provides a simple, convenient, and rapid method for detecting soil N-acetyl-β-D-glucosaminidase activity. The principle is based on S-NAG hydrolyzing N-acetyl-β-D-glucosaminide to generate p-nitrophenol, which exhibits a maximum absorption peak at 400 nm. The activity of NAG is calculated by measuring the change in absorbance at 400 nm.