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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤β-木糖苷酶(S-β-XYS)存在于植物、细菌和真菌等生物体,是催化木聚糖类半纤维素降解的关键酶,产物木糖可作为碳源应用于微生物发酵。另外,β-木糖苷酶还可以作为生物漂白剂应用于造纸工业,比传统的漂白法环保,具有广泛的应用价值。该产品提供了一种简单、方便、快速的土壤β-木糖苷酶活性检测方法。其原理是β-木糖苷酶催化对硝基苯酚-β-D-木糖苷产生对硝基苯酚,对硝基苯酚在 405 nm 处有特征吸收峰,测定405nm光吸收增加速率,可计算β-木糖苷酶活性。
Soil β-xylosidase (S-β-XYS) is present in organisms such as plants, bacteria, and fungi. It is a key enzyme that catalyzes the degradation of hemicellulose such as xylan, and the product xylose can be used as a carbon source in microbial fermentation. In addition, β-xylosidase can also be used as a biological bleaching agent in the paper industry, which is more environmentally friendly than traditional bleaching methods and has broad application value. This product provides a simple, convenient, and rapid method for detecting soil β-xylosidase activity. The principle is that β-xylosidase catalyzes the production of p-nitrophenol from p-nitrophenyl-β-D-xyloside. p-Nitrophenol has a characteristic absorption peak at 405 nm. By measuring the rate of increase in absorbance at 405 nm, the β-xylosidase activity can be calculated.