计算溶液所需的质量、体积或浓度。
BioReagent,即用型,用于蛋白分析,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 425 篇同行评审文献引用。
硫酸铵(Ammonium sulfate, CAS: 7783-20-2, 分子量 132.14, 化学式 (NH₄)₂SO₄)配制的饱和硫酸铵溶液(SAS)是蛋白质分离纯化中常用的沉淀试剂,广泛用于硫酸铵盐析工艺。
盐析原理:高浓度硫酸铵解离产生的铵根离子与硫酸根离子可竞争性结合水分子,破坏蛋白质表面水合层,降低蛋白质溶解度,使目标蛋白从体系中沉淀析出。
使用建议:硫酸铵在水中的溶解度受温度影响相对平缓,是其作为盐析试剂的优势;但饱和硫酸铵溶液的实际饱和度仍会随温度小幅变化,低温储存时可能析出晶体。建议使用前将溶液回温至室温,确认仍为饱和状态,并重新检测 pH,按需调节。
操作步骤(仅供参考):
按实验具体要求操作。
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。
2、本产品仅供科研使用,严禁它用。
Protocol (for reference only):Saturated ammonium sulfate solution (SAS), prepared from ammonium sulfate (CAS: 7783-20-2, molecular weight: 132.14, chemical formula: (NH₄)₂SO₄), is a common precipitant widely used in ammonium sulfate salting-out for protein isolation and purification.
Salting-out principle: Ammonium and sulfate ions dissociated from high-concentration ammonium sulfate competitively bind water molecules, disrupting the hydration shell on protein surfaces, reducing protein solubility and causing target proteins to precipitate out of the solution.
Usage recommendations: The solubility of ammonium sulfate in water changes relatively gradually with temperature, which is an advantage of its use as a salting-out reagent; however, the actual saturation level of a saturated ammonium sulfate solution may still vary slightly with temperature, and crystals may precipitate during low-temperature storage. Before use, it is recommended to warm the solution back to room temperature, confirm that it remains saturated, re-measure the pH, and adjust as needed.
Protocol (for reference only):
Follow the specific experimental requirements.
Precautions:
1.For your safety and health, please wear a lab coat and disposable gloves during operation.
2.For research use only. Not for clinical use.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1528792 | |
| 分析证书 | S1528792 |
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