J2130679计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品介绍
Super Green I（20× DMSO溶液，PCR级）（效果与Sybr Green I一样） 与dsDNA 结合荧光信号可增强800～1000倍。在PCR反应体系中，加入过量Super Green I荧光染料，它特异性地掺入DNA双链后，荧光信号增强，而不掺入链中的SUPER Green I染料分子荧光不变，从而保证荧光信号的增加与PCR产物的增加完全同步。荧光可以在退火阶段或者延伸阶段测定。
1．使用浓度对荧光PCR结果的影响
Super Green I的使用浓度是保证荧光定量PCR实验成功非常关键的因素。如果SUPER Green I的浓度过低会使荧光信号的变化降低，这就意味着低拷贝的样品可能无法检出。而在高浓度时，将会抑制PCR反应，降低PCR反应效率。所以一般在使用SUPER Green I时应根据实际情况优化使用浓度，反应的终浓度为1×到0.2×之间。
2．Mg2＋浓度的影响
提高Mg2＋浓度可以降低SUPER Green I对PCR反应的抑制作用。我们建议在用SUPER Green I进行荧光PCR反应时，Mg2＋浓度比无SUPER Green I 的普通 PCR 反应高出0.5～3mM。
产品参数
Ex(nm)# | 497 | Em(nm)# | 525 |
Product Description
Super Green I (20× DMSO solution, PCR grade) (the effect is the same as Sybr Green I) Combining with dsDNA, the fluorescence signal can be enhanced 800-1000 times. In the PCR reaction system, an excessive amount of Super Green I fluorescent dye is added. After it is specifically incorporated into the DNA double-strand, the fluorescent signal is enhanced, while the fluorescence of the SUPER Green I dye molecule that is not incorporated in the chain remains unchanged, thereby ensuring the fluorescence signal The increase is completely synchronized with the increase of PCR products. Fluorescence can be measured in the annealing stage or the extension stage.
1. The influence of concentration on the result of fluorescence PCR
The concentration of Super Green I is a very critical factor to ensure the success of fluorescent quantitative PCR experiments. If the concentration of SUPER Green I is too low, the change in fluorescence signal will be reduced, which means that low-copy samples may not be detected. At high concentrations, the PCR reaction will be inhibited and the PCR reaction efficiency will be reduced. Therefore, when using SUPER Green I, the concentration should be optimized according to the actual situation. The final concentration of the reaction is between 1× and 0.2×.
2. The influence of Mg2＋ concentration
Increasing the concentration of Mg2+ can reduce the inhibitory effect of SUPER Green I on the PCR reaction. We recommend that when using SUPER Green I for fluorescent PCR reactions, the Mg2+ concentration is 0.5-3 mM higher than that of ordinary PCR reactions without SUPER Green I.
Product Parameter
Ex(nm)# | 497 | Em(nm)# | 525 |
规格
规格或纯度
20× DMSO 溶液, PCR
英文名称
Super Green I for PCR
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）