计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Simmiparib is a highly potent and orally active PARP1 and PARP2 inhibitor with IC 50 values of 1.75 nM and 0.22 nM, respectively. Simmiparib has more potent PARP1/2 inhibition than its parent Olaparib . Simmiparib induces DNA double-strand breaks (DSB) accumulation and G2/M arrest in homologous recombination repair (HR)-deficient cells, thereby inducing apoptosis . Simmiparib exhibits remarkable anticancer activities in cells and nude mice bearing xenografts
In Vitro
Simmiparib (0-10 μM; 3 days) exhibits anti-proliferative activity against various cancer cells. Simmiparib (0-10 μM; 48 h) induces typical G2/M arrest in Capan-1 cells. Simmiparib (0.1-2 μM; 24 h) induces apoptosis in MDA-MB-436 and V-C8 (BRCA2 -/- ) cells, and increases dose-dependently the levels of γH2AX. Simmiparib (1-10 μM; 48 h or 72 h) increases the phosphorylation levels of Chk1 and Chk2 and the protein levels of p-Cyclin B1 (S147), Cyclin B1, p-CDK1 (Y15) and CDK1. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: Various cancer cells harboring deficient BRCA1, BRCA2, PTEN and EWS-FLI1 Concentration: 0-10 μM Incubation Time: 3 days Result: Exhibited anti-proliferative activity against MDA-MB-436 (BRCA1 -/- ), RD-ES (EWS-FLI1), DoTc2-4510 (BRCA2 -/- ), Capan-1 (BRCA2 -/- ) and U251 (PTEN -/- ) with IC 50 s of 0.2 nM, 4.6 nM, 20 nM, 21 nM and 36 nM, respectively. Cell Cycle AnalysisCell Line: Capan-1 cells Concentration: 0, 1, 3 and 10 μM Incubation Time: 48 h Result: Induced typical G2/M arrest in a concentration-dependent manner. Apoptosis AnalysisCell Line: MDA-MB-436 Concentration: 0.1 and 1 μM Incubation Time: 24 h Result: Led to 39.64% and 42.98% apoptosis at 0.1 and 1 μM, respectively. Increased dose-dependently the levels of γH2AX. Apoptosis AnalysisCell Line: V-C8 (BRCA2 -/- ) Concentration: 0.5 and 2 μM Incubation Time: 24 h Result: Caused more than 57% apoptosis. Western Blot AnalysisCell Line: Capan-1 Concentration: 1 and 10 μM Incubation Time: 48 h or 72 h Result: Increased the phosphorylation levels of Chk1 and Chk2 but did not change the levels of the corresponding total proteins. Increased the protein levels of p-Cyclin B1 (S147), Cyclin B1, p-CDK1 (Y15) and CDK1.
In Vivo
Simmiparib (2, 4 and 8 mg/kg; p.o.; qd, for 14 days) inhibits the growth of tumor in V-C8 (BRCA2 -/- ) and MDA-MB-436 (BRCA2 -/- ) xenograft mice models . Simmiparib (10 and 50 mg/kg; p.o.; qd, for 42 days) inhibits the growth of BRCA1-mutated breast cancer in xenograft mice model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female BALB/cA nude mice (Subcutaneously injected with BRCA2 -/- V-C8 cells and BRCA2 -/- MDA-MB-436 cells) Dosage: 2, 4 and 8 mg/kg Administration: p.o.; qd, for 14 days Result: Apparently inhibited the growth of the V-C8 tumor with an inhibition rate of 74.53% at 8 mg/kg. Suppressed the growth of the BRCA1-deficient MDA-MB-436 xenografts in a dose-dependent manner with its average inhibition rates of 64.93, 82.98 and 85.79% at 2, 4 and 8 mg/kg. Did not cause significant loss of body weight. Animal Model: Female BALB/cA nude mice (Subcutaneously injected with cancer cells derived from BRCA1-mutated BR-05-0028 breast cancer tissue) Dosage: 10 and 50 mg/kg Administration: p.o.; qd, for 42 days Result: Elicited dose-dependent growth inhibition with the inhibition rate of 76.73% and 93.82% at 10 mg/kg and 50 mg/kg, respectively.
Form:Solid
IC50& Target:PARP1 0.74 nM (IC 50 ) PARP2 0.22 nM (IC 50 )
规格
别名
辛米帕尼
规格或纯度
≥99%
英文名称
Simmiparib
生化机理
Simmiparib是一种高效口服活性PARP1和PARP2抑制剂，其IC 50值分别为1.75 nM和0.22 nM。Simmiparib 对 PARP1/2 的抑制作用比其母体 Olaparib（HY-10162）更强。Simmiparib可诱导DNA双链断裂。
储存条件
-20°C储存
运输条件
超低温运输
纯度
≥99%
名称和识别符
分子类型
小分子
Smiles
O=C1NN=C(CC2=CC=C(F)C(C(N3CC4=NN=C(C(F)(F)F)N4C(C)C3)=O)=C2)C5=C1C=CC=C5
分子量
486.42
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 100 mg/mL (205.58 mM; ultrasonic and warming and heat to 60°C)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥99%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 1551355-46-4，分子式为 C23H18F4N6O2，分子量为 486.42 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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