I2628570计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
阿拉丁生产的SDS-PAGE快速电泳液(Tris-Gly, 20X) (SDS-PAGE Rapid Electrophoresis Buffer for Tris-Gly, 20X)是一种用于最常用的Tris-Gly缓冲系统的SDS-PAGE快速电泳缓冲液。在保证电泳效果的前提下，用 220V 电压 20min 即可完成电泳。相比传统的电泳缓冲液，节省一个小时以上的电泳时间。显著加快电泳过程， 提高试验效率。 电泳时当电泳缓冲液温度超过 40℃时，请使用冰浴。本产品为20倍浓缩(20X)溶液，使用前需要用水稀释至1X。
产品特点
1.快速，当电泳缓冲液温度超过 40℃时，请使用冰浴。建议使用电压为 200V。
2.条带清晰。
3.操作简单，无需称量和过滤试剂。
注意事项
1.1× 工作液配制好后使用时间不宜超过 2 周。（密封保存可延长至 1 个月）
2.高电压电泳，温度较高，凝胶容易产生气泡，不影响电泳。当温度超过 40℃时，请使用冰浴。
3.仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
4.为了您的安全和健康，请穿戴好个人防护装备和服装进行操作。
使用说明
超快电泳缓冲液和传统电泳缓冲液的使用方法相同。
以下是结合 Quick-Bands和荧光蛋白分子量标准的使用手册：
1.准备样品，使用荧光上样缓冲液
使用荧光上样缓冲液 Quick-Bands处理蛋白样品。100℃或沸水浴加热 5-10 分钟，以充分
变性蛋白。
2.选择合适浓度的蛋白电泳凝胶
2.1.选择预制胶：
参考凝胶迁移率图谱根据蛋白分子量的大小选择合适浓度的 Tris-gly 体系的预制胶。
2.2.凝胶配制试剂盒：
参考说明书使用试剂盒配制实验所需规格的凝胶。
3.上样：蛋白样品冷却到室温后，直接上样到 SDS-PAGE 胶加样孔内即可。
4.配制 1× 电泳缓冲液
配制
①5×：量取 100mL 母液，倒入到一洁净的烧杯中，加入蒸馏水或去离子水至约 400mL，混匀。
②10×：量取 50mL 母液，倒入到一洁净的烧杯中，加入蒸馏水或去离子水至约 400mL，混匀。
在量筒中定容到 500mL，即为 1× 工作液。
混匀后即可使用。
5.电泳：调整电泳仪为恒压 220V，进行电泳操作，约 20min 完成蛋白电泳实验。
200V-220V 电压下电泳，20min 左右电泳结束，由于高电压电泳，温度较高，凝胶容易产生气泡，不影响电
泳。电泳时当电泳缓冲液温度超过 40℃时，请使用冰浴。
电泳实验完成以后，使用荧光上样缓冲液处理后的样品，可直接通过紫外灯、LED 灯或凝胶成像系统进行拍
照观察和分析，无需拆胶、染色和脱色。Quick-Bands 的灵敏度高于银染。
Product introduction：SDS-PAGE Rapid Electrophoresis Buffer for Tris-Gly ( 20X ) produced by Aladdin is the most commonly used Tris-Gly buffer system for SDS-PAGE. Under the premise of ensuring the electrophoresis effect, the electrophoresis can be completed with 220 V voltage for 20 min. Compared with the traditional electrophoresis buffer, save more than an hour. Electrophoresis time. Significantly accelerate the electrophoresis process, improve test efficiency. When electrophoresis buffer temperature exceeds 40 °C, please use ice bath.
Product characteristic：
1.Rapid, when the electrophoresis buffer temperature exceeds 40 °C, please use the ice bath. It is recommended to use a voltage of 200 V.
2. The stripes are clear.
3.Simple operation, no need to weigh and filter reagents.
Matters needing attention：
1.1 × working fluid should not be used for more than 2 weeks after preparation. ( Sealed preservation can be extended to 1 month )
2.High voltage electrophoresis, high temperature, the gel is easy to produce bubbles, does not affect the electrophoresis. When the temperature exceeds 40 °C, please use an ice bath.
3. It shall be used only for scientific research by professionals, not for clinical diagnosis or treatment, not for food or medicine, and not for storage in ordinary houses.
4.For your safety and health, please wear personal protective equipment and clothing to operate.
Instructions:
The usage of ultrafast electrophoresis buffer is the same as that of traditional electrophoresis buffer.
The following is a user manual that combines Quick Bands and fluorescent protein molecular weight standards:
1. Prepare the sample and use fluorescent loading buffer
Use fluorescent loading buffer Quick Bands to process protein samples. Heat at 100 ℃ or in a boiling water bath for 5-10 minutes to ensure sufficient heating
Denaturated protein.
2. Select the appropriate concentration of protein electrophoresis gel
2.1. Choose prefabricated adhesive:
According to the mobility map of gel, select the appropriate concentration of Tris gly system preform according to the size of protein molecular weight.
2.2. gel preparation kit:
Refer to the instructions and use the kit to prepare gel of the required specification for the experiment.
3. Sample loading: After cooling the protein sample to room temperature, it can be directly loaded into the SDS-PAGE gel well.
4. Prepare 1 x electrophoresis buffer solution
Preparation
① 5 ×: Measure 100mL of mother liquor and pour it into a clean beaker. Add distilled water or deionized water to about 400mL and mix well.
② 10 ×: Measure 50mL of mother liquor and pour it into a clean beaker. Add distilled water or deionized water to about 400mL and mix well.
Fill the measuring cylinder to 500mL, which is 1 x working fluid.
After mixing well, it can be used.
5. Electrophoresis: Adjust the electrophoresis instrument to a constant voltage of 220V, perform electrophoresis operation, and complete the protein electrophoresis experiment in about 20 minutes.
Electrophoresis under 200V-220V voltage is completed in about 20min. Due to high voltage electrophoresis and high temperature, gel is easy to produce bubbles, which does not affect the electrical conductivity
Swimming. When the temperature of the electrophoresis buffer exceeds 40 ℃ during electrophoresis, please use an ice bath.
After the electrophoresis experiment is completed, the samples treated with fluorescent loading buffer can be photographed directly through ultraviolet lamp, LED lamp or gel imaging system
According to observation and analysis, there is no need to remove the glue, dye or decolorize. The sensitivity of Quick Bands is higher than that of silver staining.
规格
产品名称
SDS-PAGE快速电泳液
规格或纯度
Tris-Gly, 20X
分子类型
未知
储存与运输
物理形态
液体
浓度
Tris-Gly, 20X
储存条件
室温
运输条件
常规运输
稳定性与储存
室温保存，一年有效。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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