SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer does not contain reducing agents such as DTT/B-mercaptoethanol, which does not affect the disulfide bond in the chain and the disulfide bond between the chains of protein molecules. But the solution contains SDS type denaturing agents. Suitable for protein sample preparation and loading in denatured non reduced SDS-PAGE. Precautions： 1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution; 2. This product is only used for scientific research and cannot be used for human experiments or treatments; 3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents. Instructions for Use： 1. Reagent preparation 1.1 Mix the protein tracer loading buffer (non reducing, 5x) with the protein sample in a ratio of 1:4; 2. Protein sample processing 2.1 Heat the protein sample in a boiling water bath for 3-5 minutes; 2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000rpm for 30 seconds: 3. Protein loading and electrophoresis 3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole; 3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.
规格
别名
变性非还原蛋白上样缓冲液 | 变性非还原PAGE蛋白上样缓冲液( 5x)
英文别名
enaturated PAGE protein loading buffer( Non reducing, 5X) | Denaturated PAGE protein loading buffer | Non-denatured Gel Sample Loading Buffer
规格或纯度
BioReagent, 用于聚丙烯酰胺凝胶电泳, for western blot
稳定性与储存
Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.
ZJ25F0420291
危险品化学品经营许可证（带存储）