分子量：54-94 kDa
物理外观：Lyophilized
储存缓冲液：Lyophilized from 5mM PBS, pH 7.4
复溶(Reconstitution)：Reconstitute in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
产品描述：
链霉亲和素（Streptavidin，SA）是阿维丁链霉菌（Streptomyces avidinii）在生长过程中分泌的一种同型四聚体蛋白。同亲和素一样，一摩尔的链霉亲和素可以结合四摩尔的生物素，与生物素具有很高的亲和力。由于 SA 不含糖基，因此 SA 在检测应用中具有比亲和素更低的非特异性结合水平，已被广泛应用于酶联免疫吸附实验、免疫组织化学、时间分辨免疫荧光技术（TRFIA）、定量 PCR、单链 DNA 制备、生物分子纯化、单克隆抗体制备等生物技术领域。
但是链霉亲和素的吸附性较差，在实际应用中尤其是吸附到 NC 膜或包埋比较困难，粘性重组链霉亲和素（S-rSA）主要是解决这个问题，其不仅具有与活性有关的核心序列，而且吸附性、稳定性、溶解性等方面均优于天然 SA，尤其在 NC 膜上的吸附性远远超越天然 SA。
Slot-Blot 法 NC 膜上不同 SA 吸附及活性比较
注意：本产品仅限于科研使用。
阿拉丁链霉亲和素系列产品：
| 货号 | 产品名称 | 物理外观 | 储存缓冲液 | 分子量 | 来源 | 活性 (U/mg蛋白) | 关键特性 |
| R748131 | 重组单体链霉亲和素（r-mSA） | Lyophilized | Lyophilized from 10mM CBS, Trehalose, pH 9.2 | 15.1kDa | E.coli | ≥8 | N-His tag，pI=7.1，易洗脱 |
| S767455 | 重组增强稳定型核心链霉亲和素（r-se-cSA） | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 53.6kDa (tetramer), 13.4kDa (monomer) | E.coli | 17-19 | 核心序列优化，序列修饰，热稳定性、溶解性、活性等提升；pI=5.68 |
| S767462 | 重组粘性链霉亲和素 (r-ScSA) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 54-94kDa | E.coli | ≥14 | 增强吸附性（特别适用于NC膜），保留核心活性 |
| R767460 | 重组核心链霉亲和素 (r-cSA) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 54kDa (tetramer), 13.5kDa (monomer) | E.coli | ≥15 | 核心序列优化；C端含半胱氨酸；pI=6.04 |
| R767461 | 重组核心链霉亲和素2 (r-cSA2) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 53.2kDa (tetramer), 13.3kDa (monomer) | E.coli | ≥16 | 核心序列优化；pI=6.09 |
| R767452 | 重组核心链霉亲和素4 (r-cSA4) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 55.2kDa (tetramer), 13.8kDa (monomer) | E.coli | ≥16 | 核心序列优化，序列修饰（增加了氨基的含量），稳定性、溶解性等提升；pI=8.93 |
| R767450 | 重组长臂巯基核心链霉亲和素（r-las-cSA）
| Lyophilized | Lyophilized from 20mM HEPES, Trehalose, TCEP, pH 7.0 | 54.8kDa (tetramer), 13.7kDa (monomer) | E.coli | ≥16 | 核心序列优化，C端的半胱氨酸前添加6个柔性氨基酸延长巯基臂；pI=6.09 |
| S1527913 | 重组增强稳定型核心链霉亲和素4 (r-se-cSA4) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 55.2kDa (tetramer), 13.8kDa (monomer) | E.coli | ≥17 | 去除与活性无关的序列，核心序列优化，序列修饰，热稳定性、溶解性、活性等大幅提升；pI=8.73 |
| L1527914 | 重组 低非特异性核心链霉亲和素 (r-LNS-cSA) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 56.8kDa (tetramer), 14.2kDa (monomer) | E.coli | ≥17 | 核心序列优化，序列修饰，热稳定性、溶解性、活性等大幅提升,对序列中的氨基酸进行突变，降低了偶联过程中的非特异性吸附；pI=8.93 |
Molecular weight: 54-94 kDa
Physical appearance: Lyophilized
Buffer storage: Lyophilized from 5mM PBS, pH 7.4
Reconstitution：Reconstitute in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Product introduction:
Streptavidin (SA) is a homologous tetramer protein secreted by Streptomyces avidinii during its growth. As with avidin, one mole of streptavidin can bind four moles of biotin, and biotin has a high affinity. Because SA does not contain a sugar group, SA has a lower non-specific binding level than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence technique (TRFIA), quantitative PCR, single-strand DNA preparation, biomolecular purification, monoclonal antibody preparation and other biotechnology fields.
However, the adsorbability of streptavidin is poor, and it is difficult to adsorb or embed it into NC film in practical applications. Viscous recombinant streptavidin (S-rSA) is mainly used to solve this problem. It not only has the core sequence related to activity, but also has better adsorption, stability and solubility than natural SA. In particular, the adsorption of NC film is far more than that of natural SA.
Comparison of adsorption and activity of different SA on NC films by Slot-Blot method
Note: This product is for scientific use only.
Aladdin Streptavidin Product Series:
| Product No. | Product Name | Physical Appearance | Buffer | Molecular Weight | Source | Activity (U/mg protein) | Key Features |
| R748131 | Recombinant Monomeric Streptavidin (r-mSA) | Lyophilized | Lyophilized from 10mM CBS, Trehalose, pH 9.2 | 15.1kDa | E.coli | ≥8 | N-His tag, pI = 7.1, easy elution |
| S767455 | Recombinant Stability-enhanced Core Streptavidin (r-se-cSA) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 53.6kDa (tetramer), 13.4kDa (monomer) | E.coli | 17-19 | Optimized core sequence and sequence modification with improved thermal stability, solubility and activity; pI = 5.68 |
| S767462 | Recombinant Sticky Streptavidin (r-ScSA) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 54-94kDa | E.coli | ≥14 | Enhanced adsorption performance especially for NC membrane with retained core activity |
| R767460 | Recombinant Core Streptavidin (r-cSA) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 54kDa (tetramer), 13.5kDa (monomer) | E.coli | ≥15 | Optimized core sequence and C-terminal cysteine; pI = 6.04 |
| R767461 | Recombinant Core Streptavidin 2 (r-cSA2) | Lyophilized | Lyophilized from 5mM PBS, pH 7.4 | 53.2kDa (tetramer), 13.3kDa (monomer) | E.coli | ≥16 | Optimized core sequence; pI = 6.09 |
| R767452 | Recombinant Core Streptavidin 4 (r-cSA4) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 55.2kDa (tetramer), 13.8kDa (monomer) | E.coli | ≥16 | Optimized core sequence with sequence modification (increased amino-group content); improved stability and solubility; pI = 8.93 |
| R767450 | Recombinant Long-Arm Sulfhydryl Group Core Streptavidin (r-las-cSA) | Lyophilized | Lyophilized from 20mM HEPES, Trehalose, TCEP, pH 7.0 | 54.8kDa (tetramer), 13.7kDa (monomer) | E.coli | ≥16 | Optimized core sequence; six flexible amino acids added before C-terminal cysteine to extend thiol arm; pI = 6.09 |
| S1527913 | Recombinant Stability-enhanced Core Streptavidin 4 (r-se-cSA4) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 55.2kDa (tetramer), 13.8kDa (monomer) | E.coli | ≥17 | Removal of non-activity-related sequences, optimized core sequence and sequence modification; greatly improved thermal stability, solubility and activity; pI = 8.73 |
| L1527914 | Recombinant Low Non-Specific Core Streptavidin (r-LNS-cSA) | Lyophilized | Lyophilized from 5mM PPB, Trehalose, pH 7.4 | 56.8kDa (tetramer), 14.2kDa (monomer) | E.coli | ≥17 | Optimized core sequence with sequence modification; greatly improved thermal stability, solubility and activity; amino-acid mutations introduced to reduce non-specific adsorption during conjugation; pI = 8.93 |
Source
E. coli
ZJ25F0521496
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