计算溶液所需的质量、体积或浓度。
石碳酸复红染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
石碳酸复红染色液与改良苯酚品红染色液不同。石碳酸复红染色液主要由石碳酸、碱性品红等组成，主要用于痰涂片或拭子结核杆菌染色和革兰氏染色，较少单独使用。改良苯酚品红染色液又称卡宝品红（Carbol fuchsin）染色液，主要由苯酚、碱性品红、山梨醇、氧化剂等组成，常用于细胞核染色、染色体的固定和染色。
石碳酸复红染色液主要用于抗酸杆菌染色和革兰氏染色中，亦可单独使用。
使用说明：
一、抗酸染色：
1、接种环挑取待检样本，涂布于载玻片上，自然干燥。
2、滴加石碳酸复红染色液，染色5~10min。不必加热，蒸馏水冲洗。
3、用脱色液脱色至无红色为止。蒸馏水冲洗。
4、用亚甲基蓝染色液染色30~60s。蒸馏水冲洗。
5、 轻轻吸干水分，自然干燥。
6、油镜镜检。
二、蜡切片染色：
1、石蜡切片脱蜡至水。
2、滴加石碳酸复红染色液，染色3~5min。流水稍洗。
3、盐酸乙醇分化5~10s。流水稍洗。
4、直接二甲苯透明，树胶封片。
注意事项：
1、染色结束后，应立即显微镜下观察。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
染色结果：
| 细胞核 | 粉红色或红色 |
Carbonate Fuchsin Staining Solution differs from modified carbol fuchsin staining solution. Composed mainly of carbolic acid and basic fuchsin, carbol fuchsin staining solution is primarily applied to sputum smear or swab staining of Mycobacterium tuberculosis and Gram staining, and rarely used alone. Also known as Carbol fuchsin, modified carbol fuchsin staining solution consists of phenol, basic fuchsin, sorbitol and oxidants, commonly used for cell nucleus staining as well as fixation and staining of chromosomes.
Carbol fuchsin staining solution is mainly used for acid-fast bacilli staining and Gram staining, and can also be used independently.
Instructions for Use:
1. Acid-fast Staining:
① Collect the test sample with an inoculating loop, spread it evenly on a glass slide and air-dry naturally.
② Add carbol fuchsin staining solution and stain for 5 to 10 minutes without heating, then rinse with distilled water.
③ Decolorize with decolorizing solution until no red color remains, followed by distilled water rinsing.
④ Stain with methylene blue staining solution for 30 to 60 seconds and rinse with distilled water.
⑤ Blot moisture gently and air-dry the specimen.
⑥ Examine under an oil immersion objective.
2. Paraffin Section Staining:
① Deparaffinize paraffin sections and rehydrate with water.
② Apply carbol fuchsin staining solution and stain for 3 to 5 minutes, then rinse briefly with running water.
③ Differentiate with hydrochloric acid ethanol for 5 to 10 seconds and rinse slightly with running water.
④ Clear with xylene directly and mount with neutral balsam.
Precautions:
1. Microscopic observation shall be conducted immediately after staining.
2. Wear lab coat and disposable gloves during operation for personal safety and health protection.
Staining Result:
| Cell Nucleus | Pink or Red |
规格
英文别名
Stone carbonate red dyeing solution | Carbonate fuchsin stain | Carbonic Acid Red Staining Solution
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
Store at room temperature long term (18 months). Store in the dark.
英文名称
Carbonate Fuchsin Staining Solution
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0636547 |
分析证书 | S774539 |
| ZJ25F0521767 |
分析证书 | S774539 |
技术文档和文章
溶液计算器
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