计算溶液所需的质量、体积或浓度。
Transwell 染色试剂盒
有货
为什么选择此级别
BioReagent,用于显微镜,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Transwell 染色试剂盒是针对 Transwell 小室培养细胞（如迁移、侵袭实验后）设计的专用染色工具，操作简便、染色精准，核心用于观察细胞形态和计数。染色液能快速渗透 Transwell 膜，不破坏细胞形态，且不易脱染，染色效果稳定。操作门槛低，无需复杂前处理，通常为 “固定→染色→清洗” 三步流程，耗时短，适合批量样本处理。可用于肿瘤细胞迁移 / 侵袭实验、内皮细胞穿透实验等，兼容常见细胞类型（如肿瘤细胞、干细胞、上皮细胞）。
| 项目号 | Components | 20T | Storage |
| T1506001A | 细胞固定液 | 50 mL | 2-8℃ |
| T1506001B | 染色液 | 20 mL | 2-8℃. Store in the dark. |
| T1506001C | 调色液A | 20 mL | 2-8℃ |
| T1506001D | 调色液B | 5 mL | 2-8℃ |
| T1506001E | 封片液 | 2 mL | 2-8℃ |
| T1506001F | PBS粉 | 1L | 2-8℃ |
注：表中20T对应的溶液体积按照6孔板定量。
自备耗材：
如果用正置显微镜观察，需要准备载玻片、盖玻片、吸头。
倒置显微镜观察，需准备吸头。
染色步骤(仅供参考)：
a.取1L双蒸水，把PBS全部溶解，后面备用。配制后可以4度保存。
b.取出准备好的细胞，吸出培养基，pbs清洗3次。
c.用棉签搽去上室面细胞，取2ml编号A液固定液加入孔板，放入transwell，固定15分钟。
d.注意：Transwell需全侵泡在液体中。
e.Pbs洗3次，每次5分钟。
f.在孔板中加入1ml编号B试剂染色液，使transwell侵泡在液体中，染色10分钟。
g.弃掉染色液，加入1mlC液，加一滴D液，快速混匀，显微镜下观察。
h.注意：D液不能直接滴到染色的细胞上；根据自己细胞染色的颜色，自行调节加入D调色液；如果颜色比较深，可以再加一滴D调色液，直到调为自己认可的颜色。
i.达到满意结果后，弃掉所有液体，用PBS清洗1次。
j.正置显微镜观察：用刀片取下膜，放在载玻片上，加1-2滴编号为E的液体封片液，盖上盖玻片,显微镜下观察。
k.倒置显微镜观察：直接把Transwell放在孔板里，此方法无法长时间保存，做完实验后立即照相。
注意事项：
1.第一次使用本试剂时建议先取1-2个样品做预实验，确定一个好染色时间。
2.如果用调色液，未达到想要的颜色，可以重复染色，重新调色，直到满足自己的实验要求。
3.为了您的安全和健康，请穿实验服并戴一次性手套、口罩操作。
细胞染色结果：
The Transwell Staining Kit is a specialized staining tool designed for cells cultured in Transwell chambers (e.g., after migration or invasion experiments). It features easy operation and precise staining, and is mainly used for observing cell morphology and counting cells. The staining solution can quickly penetrate the Transwell membrane without damaging cell morphology. It also has good stain retention (not easy to fade) and provides stable staining results. With a low operational threshold, the kit requires no complex pretreatment. Its procedure typically consists of three steps: "fixation → staining → washing". It takes little time and is suitable for batch sample processing. This kit can be used in tumor cell migration/invasion experiments, endothelial cell penetration experiments, etc., and is compatible with common cell types (such as tumor cells, stem cells, and epithelial cells).
| T1506001 | Components | 20T | Storage |
| T1506001A | Cell Fixative | 50 mL | 2-8℃ |
| T1506001B | Staining Solution | 20 mL | 2-8℃. Store in the dark. |
| T1506001C | Toning Solution A | 20 mL | 2-8℃ |
| T1506001D | Toning Solution B | 5 mL | 2-8℃ |
| T1506001E | Mounting Medium | 2 mL | 2-8℃ |
| T1506001F | PBS (powder) | 1L | 2-8℃ |
Note: The solution volume corresponding to 20T in the table is quantified based on the 6-well plate.
Self-Prepared Consumables:
If observing with an upright microscope, slides, coverslips, and pipette tips need to be prepared.
If observing with an inverted microscope, only pipette tips need to be prepared.
Staining Procedures (For Reference Only):
a. Take 1 L of double-distilled water, dissolve all PBS in it, and set aside for later use. The prepared solution can be stored at 4°C.
b. Take out the prepared cells, aspirate the culture medium, and rinse the cells with PBS 3 times.
c. Wipe off the cells on the upper chamber surface with a cotton swab, add 2 mL of Fixative (Solution A, numbered) to the well plate, place the Transwell into the well plate, and fix for 15 minutes.
d. Note: The Transwell must be fully immersed in the liquid.
e. Rinse with PBS 3 times, 5 minutes each time.
f. Add 1 mL of Staining Solution (Reagent B, numbered) to the well plate, ensure the Transwell is immersed in the liquid, and stain for 10 minutes.
g. Discard the staining solution, add 1 mL of Solution C, then add one drop of Solution D, mix quickly, and observe under a microscope.
h. Notes: Solution D must not be dropped directly onto the stained cells. Adjust the amount of Solution D (toning solution) added according to the staining color of your cells. If the color is too dark, add another drop of Solution D until the color meets your satisfaction.
i. After achieving a satisfactory result, discard all liquids and rinse once with PBS.
j. Observation with an upright microscope: Use a blade to remove the membrane, place it on a glass slide, add 1–2 drops of Mounting Medium (Solution E, numbered), cover with a coverslip, and observe under the microscope.
k. Observation with an inverted microscope: Place the Transwell directly in the well plate. This method does not allow long-term storage, so take photos immediately after completing the experiment.
Precautions:
1. When using this reagent for the first time, it is recommended to take 1-2 samples for a preliminary experiment to determine an optimal staining time.
2. If the desired color is not achieved when using the toning solution, you can repeat the staining and toning processes until your experimental requirements are met.
3. For your safety and health, please wear a lab coat, disposable gloves, and a face mask during operation.
Cell Staining Results:
规格
别名
细胞小室染色试剂盒
英文别名
Transwell Cell Staining Kit | Cell Migration And Invasion Stain Kit (For Transwell) | Cell Chamber Staining Kit
规格或纯度
BioReagent,用于细胞培养,用于显微镜
英文名称
Transwell Staining Kit
应用
Cell Staining
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131346 |
分析证书 | T1506001 |
技术文档和文章
溶液计算器
常见问题
什么是「用于显微镜」？
「用于显微镜」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。
什么是「BioReagent」？
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本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
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