神经元细胞体包括一个具有皱褶核膜的大细胞核、稀疏的染色质和一个明显的核仁。在细胞体中细胞质是尼氏颗粒。尼氏颗粒可以用很多染色来显示如中性红、亚甲基蓝、甲苯胺蓝和甲基紫等。染色的变异、pH和分化的时间使一些染色既可以仅突出尼氏物质,也可以显示神经元的细胞核和神经胶质。尼氏体 (Nissl body)或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质,能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成紫蓝色。各种神经细胞内都含有尼氏体,但其形状、数量、分布位置常常不同。尼氏体也存在于树突中,但不在于轴突和包体的轴丘。尼氏体因为生理状态的变化而变化,尼氏体是神经元内合成蛋白质合成的重要部位,当神经元受到刺激后,包体内的尼氏体会明显减少。
注意事项:
1、尼氏小体染色尼氏小体呈紫色,背景接近于无色,染色后避光保存。
2、本产品仅供科研使用,严禁它用。
The neuronal cell body contains a large cell nucleus with a folded nuclear membrane, sparse chromatin, and a prominent nucleolus. Within the cell body, the cytoplasm contains Nissl granules. Nissl granules can be visualized using various stains, such as neutral red, methylene blue, toluidine blue, and methyl violet. Variations in staining, pH levels, and differentiation time allow some stains to either highlight only Nissl substances or display both the cell nuclei of neurons and neuroglia. Nissl bodies (also known as Nissl granules) are triangular or oval-shaped small masses distributed in the cytoplasm of nerve cells. They can be stained purple-blue by basic dyes, including thionine, methylene blue, toluidine blue, and cresyl violet. All types of nerve cells contain Nissl bodies, but their shape, quantity, and distribution location often vary. Nissl bodies are also present in dendrites, but not in axons or the axon hillock of the cell body. Nissl bodies change according to the physiological state of the neuron. As an important site for protein synthesis in neurons, the number of Nissl bodies in the cell body decreases significantly when the neuron is stimulated.
Precautions
1. For Nissl body staining: Nissl bodies appear purple, while the background is nearly colorless. After staining, store the sample away from light.
2. This product is for research use only. Any other uses are strictly prohibited.