尼氏体 (Nissl body)或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质,能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成蓝色。焦油紫染色液 (0.1%)以进口焦油紫作为核心染料,焦油紫具有感光作用,能够很好地显示尼氏体的变化。本试剂操作简单、染色稳定、适用范围广,可用于石蜡组织切片的尼氏物质、神经元等的染色。尼氏体的存在和消失是神经细胞是否受损的重要指标,当发生脑炎、脑缺血、轴突反应等情况,尼氏体会发生溶解,甚至消失。
操作步骤(仅供参考):
1、新鲜组织固定于乙醇、Carnoy固定液或中性福尔马林溶液,常规脱水包埋。
2、切片厚5μm,常规脱蜡至水。
3、切片放入Cresyl violet Stain,将染色缸置于温箱浸染,酒精灯上加温使切片冒气泡为止 (大约10min)。
4、蒸馏水冲洗。
5、加入70%乙醇或Nissl Differentiation分化,在显微镜下观察至背景接近于无色为止。
6、无水乙醇迅速脱水。二甲苯透明,中性树胶封固。
染色结果:
尼氏体:紫色
背景:接近于无色
注意事项:
1、尼氏体离体后容易溶解,所以组织取出后应立即固定,否则难以着色。
2、组织固定起着非常重要的作用,固定可采用乙醇、Carnoy固定液或中性福尔马林溶液。
3、本染色试剂盒对石蜡组织切片的尼氏染色效果较好。
4、石蜡切片厚度7-10μm或25μm (皮质神经元密度的评估要用25μm厚的切片)。
5、染色后的标本务必避光保存,否则容易褪色。
6、本产品仅供科研使用,严禁它用。
Nissl bodies (also called Nissl granules) are triangular or oval small masses distributed in the cytoplasm of nerve cells. They can be stained blue by basic dyes such as thionine, methylene blue, toluidine blue, and cresyl violet. Cresyl Violet Staining Solution (0.1%) uses imported cresyl violet as the core dye. Cresyl violet has a photosensitive effect and can well display changes in Nissl bodies. This reagent features simple operation, stable staining, and a wide range of applications. It can be used for staining Nissl substances, neurons, etc., in paraffin-embedded tissue sections. The presence or absence of Nissl bodies is an important indicator of whether nerve cells are damaged. When conditions such as encephalitis, cerebral ischemia, or axonal reaction occur, Nissl bodies will dissolve or even disappear.
Operating Procedures (for Reference Only)
1. Fix fresh tissues in ethanol, Carnoy's fixative, or neutral formalin solution, then perform routine dehydration and embedding.
2. Cut sections with a thickness of 5 μm, and conduct routine dewaxing to water.
3. Place the sections into Cresyl Violet Stain, put the staining jar in an incubator for impregnation, and heat with an alcohol lamp until the sections start to bubble (approximately 10 minutes).
4. Rinse with distilled water.
5. Add 70% ethanol or Nissl Differentiation solution for differentiation, and observe under a microscope until the background is nearly colorless.
6. Dehydrate quickly with absolute ethanol, clear with xylene, and mount with neutral gum for sealing.
Staining Results
Nissl bodies: Purple
Background: Nearly colorless
Precautions
1. Nissl bodies are prone to dissolution after being isolated from the body. Therefore, tissues should be fixed immediately after removal; otherwise, staining will be difficult.
2. Tissue fixation plays a crucial role. Ethanol, Carnoy's fixative, or neutral formalin solution can be used for fixation.
3. This staining kit provides good results for Nissl staining of paraffin-embedded tissue sections.
4. The thickness of paraffin sections can be 7-10 μm or 25 μm (25 μm-thick sections are required for evaluating the density of cortical neurons).
5. Stained specimens must be stored away from light; otherwise, they are prone to fading.
6. This product is for research use only. Any other uses are strictly prohibited.