计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胆固醇(Cholesterol)又称胆甾醇,是一种环戊烷多氢菲的衍生物,广泛存在于动物体内,其中脑、神经组织最丰富,在肾、脾、皮肤、肝和胆汁中含量也较高,用酶学方法测定总胆固醇(Total Cholesterol,TC)是生化检测中的常用方法。 血液中的胆固醇约1/3为游离胆固醇,2/3为与脂肪酸结合的胆固醇酯,后者被胆固醇酯酶(CEH)水解为游离胆固醇,游离胆固醇被胆固醇氧化酶(COD)氧化成胆甾烯酮,并产生过氧化氢,再经过氧化物酶(POD)催化,使4-氨基安替比林与酚(三者合称PAP)反应,生成红色醌亚胺色素(Trinder反应),分光光度计在500~520nm处进行比色测定。 该试剂盒主要用于人或动物的血清、血浆、脑脊液、细胞、组织等样本中的总胆固醇含量定量测定。仅用于科研领域,不适用于临床诊断或其他用途。
Cholesterol, also known as cholesterin, is a derivative of cyclopentanoperhydrophenanthrene widely distributed in animals. It is most abundant in the brain and nervous tissue, with relatively high levels also found in the kidneys, spleen, skin, liver, and bile. The enzymatic method for determining Total Cholesterol (TC) is a common procedure in biochemical testing. In blood, approximately one-third of cholesterol exists in the free form, while the remaining two-thirds is esterified with fatty acids. In the assay, cholesterol esterase (CEH) hydrolyzes cholesterol esters into free cholesterol. The free cholesterol is then oxidized by cholesterol oxidase (COD) to form cholestenone, with the concomitant production of hydrogen peroxide. Subsequently, in a reaction catalyzed by peroxidase (POD), the hydrogen peroxide facilitates the reaction of 4-aminoantipyrine with phenol (collectively termed PAP) to generate a red-colored quinoneimine dye (the Trinder reaction). This product is quantified colorimetrically using a spectrophotometer at a wavelength between 500 and 520 nm. This assay kit is primarily designed for the quantitative determination of total cholesterol content in samples such as human or animal serum, plasma, cerebrospinal fluid, cells, and tissues. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.