计算溶液所需的质量、体积或浓度。
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Tris - 乙酸电泳缓冲液(Tris-Acetate-EDTA Buffer,简称TAE),是实验室最常用的DNA 电泳缓冲液。
1×TAE 工作液标准组成为:40 mM Tris-Acetate、1 mM EDTA(pH 8.0)。
TAE 缓冲液 (50×, 粉剂) 主要由 2 M Tris-Acetate、50 mM EDTA 组成,使用前用蒸馏水或去离子水溶解配制成 50× 浓缩液,稀释至 1× 后作为工作液使用。
本试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
1. 50×TAE 浓缩液配制
取 700 mL 蒸馏水或去离子水,依次加入试剂 A 和试剂 B,充分搅拌至完全溶解,再补水定容至 1000 mL,即得到 50×TAE 缓冲液。
2. 工作液使用
将配制好的 50×TAE 缓冲液稀释至1×,即可用于 DNA 电泳实验。
注意事项
1. 试剂 A 为粉剂;试剂 B 常温下为液体,低温下可呈固态,可水浴加热助溶后使用。
2. 若单次使用量较小,建议试剂分装后再使用,减少反复开盖污染。
3. 操作时请穿戴实验服及一次性手套,保障实验安全。
4. 试剂开封后请尽快使用并密封保存,避免影响后续实验效果。
Tris-Acetate-EDTA Buffer (TAE) is the most commonly used buffer for DNA electrophoresis in laboratories.
The standard composition of 1× TAE working solution is: 40 mM Tris-Acetate, 1 mM EDTA (pH 8.0).
TAE Buffer (50×, Powder) is primarily composed of 2 M Tris-Acetate and 50 mM EDTA. Before use, it should be dissolved in distilled or deionized water to prepare a 50× concentrated stock solution. This stock solution is then diluted to 1× for use as the working solution.
This reagent is for research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
1. Preparation of 50× TAE Concentrated Stock Solution
Take approximately 700 mL of distilled or deionized water.
Add Reagent A and Reagent B sequentially, stirring thoroughly until completely dissolved.
Bring the final volume up to 1000 mL with water. This yields the 50× TAE Buffer stock solution.
2. Preparation of Working Solution
Dilute the prepared 50× TAE Buffer stock solution to 1× for use in DNA electrophoresis experiments.
Notes
1. Reagent A is provided as a powder. Reagent B is liquid at room temperature but may solidify at lower temperatures; it can be dissolved by warming in a water bath if needed.
2. If the single-use volume is small, it is recommended to aliquot the reagent after preparation to reduce contamination risk from repeated opening.
3. Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.
4. Use the reagent as soon as possible after opening and keep it sealed to avoid affecting subsequent experimental results.
| T1519717 | Component | 1 L | Storage |
| T1519717A | 试剂 A | 1 EA | RT. |
| T1519717B | 试剂 B | 1 EA | RT. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T1519717 | |
| 分析证书 | T1519717 |
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