质谱级 Trypsin-N 蛋白酶特异性水解在赖氨酸 K 和精氨酸 R 片段的 N 端肽键。新的酶切 N 端的系列蛋白酶。
物理形态：质谱级 Trypsin-N 蛋白酶是冻干粉，每瓶含有 0.47 mg HEPES、0.05mg CaCl₂
溶解液: 建议使用 50mM 醋酸锌溶液 40μl 溶解后使用。
pH 值范围：在 pH=7.5 时，Trypsin-N 具有最佳活性。
适用范围: Trypsin-N 蛋白酶特异性水解变性后蛋白质 K 位和 R 位肽键。
通用溶液酶解法:
蛋白酶推荐酶切体系: 20-50mM HEPES 或 Tris 缓冲液。
使用条件：按照 20:1 蛋白和蛋白酶的量 (w/w) 使用，37℃恒温反应 4 小时以上。
温馨提示:
1. 最大活性：建议蛋白样本经过变性和烷基化处理后，同时严格执行酶切反应体系和 SOP。
2. 敏感性：对 1mM DTT, 0.1mM 盐酸胍，0.8M 尿素，5% 乙腈敏感，会降低酶切效率。
MS-grade Trypsin-N protease specifically cleaves peptide bonds at the N-terminus of lysine (K) and arginine (R) residues. It is a new series of proteases with N-terminal cleavage specificity.
Physical Form:
MS-grade Trypsin-N protease is supplied as a lyophilized powder, containing 0.47 mg HEPES and 0.05 mg CaCl₂ per vial.
Reconstitution Solution:
Reconstitute with 40 μL of 50 mM zinc acetate solution before use.
pH Range:
Trypsin-N exhibits optimal activity at pH 7.5.
Application:
Trypsin-N protease specifically cleaves peptide bonds at lysine (K) and arginine (R) residues in denatured proteins.
General In-Solution Digestion Protocol:
Recommended Digestion Buffer: 20–50 mM HEPES or Tris buffer.
Reaction Conditions: Use a protein-to-protease ratio (w/w) of 20:1. Incubate at 37°C for at least 4 hours.
Tips:
1. Maximizing Activity: It is recommended that protein samples undergo denaturation and alkylation, and that the digestion reaction system and SOP are strictly followed.
2. Sensitivity: Trypsin-N is sensitive to 1 mM DTT, 0.1 mM guanidine hydrochloride, 0.8 M urea, and 5% acetonitrile, which will reduce digestion efficiency.
规格
产品名称
N 端特异性胰蛋白酶（质谱级）
规格或纯度
EnzymoPure™,质谱级 (MS),重组,≥99%(HPLC),≥40 U/mg protein
危险品化学品经营许可证（带存储）