计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无RNA酶,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
固定的目的在于保存细胞和组织的原有形态结构,固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长。固定液分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等,较为常用的是醛类中的福尔马林、醇类中的乙醇。
组织细胞固定液 (4% PFA,RNase free) 主要由优质多聚甲醛、磷酸盐、DEPC 处理水等组成,pH 值约为 7.4,适合于绝大多数组织和细胞的固定,尤其适用于与 RNA 有关实验,它能较好的保护组织和细胞的形态结构以及 RNA。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、一般组织固定时间控制在 4~12 h,大标本应适当延长固定时间,培养细胞或细胞爬片固定时间控制在 10~15 min,特殊情况除外。
2、样本固定完毕后,可放置于 4℃环境中长期保存。
注意事项:
1、该固定液无法高压灭菌,所以无法做到彻底的 RNase free,操作时请注意该细节。
2、组织细胞固定液 (4% PFA, RNase free) 有一定刺激性和腐蚀性,一经开启,储存过久固定效果易下降。
3、避免过度延长固定时间,易引起生物大分子过度交联;取材厚度不同,固定时间也不同。
4、固定液的容量应足够,一般固定液与组织块的体积比率应大于 10:1。
5、温度对固定的影响很明显,提高温度可以加速固定作用,但温度不宜过高。
6、取出新鲜组织后,应及时固定,无法及时固定时,应保存于生理盐水中及时送检。
7、为了您的安全和健康,请穿实验服并戴一次性手套操作。
8、试剂开封后请尽快使用,以防影响后续实验效果。
The purpose of fixation is to preserve the original morphology and structure of cells and tissues. Fixatives prevent autolysis of tissues and cells induced by endogenous lysosomal enzymes and inhibit the growth of bacteria and molds. Fixatives are classified into aldehyde fixatives, mercury‑based fixatives, alcohol fixatives, oxidant fixatives, picrate fixatives and so on. Among them, formalin (aldehyde‑based) and ethanol (alcohol‑based) are the most commonly used.
Tissue‑Cell Fixative (4% PFA, RNase‑free) is mainly composed of high‑grade paraformaldehyde, phosphate and DEPC‑treated water, with a pH value of approximately 7.4. It is suitable for the fixation of most tissues and cells, especially for RNA‑related experiments, as it can well preserve the morphological structure of tissues and cells as well as RNA. This reagent is for research use only and is not intended for clinical diagnosis or other applications.
Operating Procedures (For Reference Only)
1. Fix common tissues for 4‑12 h; prolong the fixation time appropriately for large specimens. For cultured cells or cell slides, fix for 10‑15 min, unless under special circumstances.
2. After fixation is completed, samples can be stored long‑term at 4 ℃.
Precautions
1. This fixative cannot be autoclaved, so absolute RNase‑free status cannot be guaranteed. Please take this point into consideration during operation.
2. Tissue‑Cell Fixative (4% PFA, RNase‑free) is irritating and corrosive. Once opened, its fixation performance tends to decline after prolonged storage.
3. Avoid excessively long fixation time, which may cause over‑crosslinking of biomacromolecules. Fixation time varies with the thickness of collected tissues.
4. Use sufficient volume of fixative; generally the volume ratio of fixative to tissue block should be greater than 10:1.
5. Temperature exerts a notable effect on fixation. Elevated temperature accelerates fixation, yet excessive temperature should be avoided.
6. Fresh tissues shall be fixed promptly after dissection. If immediate fixation is unavailable, store tissues in normal saline for prompt delivery for processing.
7. Wear a lab coat and disposable gloves for your safety and health during operation.
8. Use the reagent as soon as possible after opening to prevent compromised experimental results.
| pH | 7.4 |
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我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 RNase free 级别指南 →