计算溶液所需的质量、体积或浓度。
Tris-Tricine-SDS(TTS)电泳缓冲液是使用Schagger和von Jagow方法对蛋白质进行SDS-聚丙烯酰胺凝胶电泳的阴极(上部储库)缓冲液。Schagger和von Jagow方法专用于分离小分子量蛋白质。它与Laemmli方法的不同之处在于使用tricine取代了甘氨酸,并且凝胶含有1M Tris-HCl,pH 8.45而不是0.375M Tris-HCl,pH8.9。将TTS缓冲液稀释10倍将获得含有100mM Tris、100mM tricine和0.1%
应用
Tris-Tricine-SDS缓冲液10×浓缩液适合用作支气管肺泡灌洗液(BALF)的聚丙烯酰胺分离的运行缓冲液。
Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).