EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品包装：
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Thermostable T7 RNA polymerase是经基因工程改造的T7 RNA聚合酶。该酶高度特异识别T7启动子序列，并且可以在50℃条件下进行高效的体外转录。在体外转录过程中，可提高加帽效率并消除dsRNA副产物的产生。
产品特点
对于T7启动子有高度的特异性，用于体外RNA（含小RNA）的合成。
产品用途
1)合成单链RNA；
2)合成高特异性RNA探针；
3)合成siRNA前体；
4)制作RNA剪接反应（RNA splicing）的前体；
5)以帽类似物（Cap analog）为引物，制作Capped mRNA。
使用建议
为了特定区域的有效转录，建议在其区域下游把模板DNA 预先切成平端或5′突出末端。
缓冲液中的亚精胺与核酸结合可能形成不溶物，建议最后加入模板DNA。
保存温度
-20℃，或-80℃长期保存。
质量保证
经多次柱纯化，SDS-PAGE胶检测仅可见清晰单一的目的条带，纯度达95%，PCR方法检测无大肠杆菌DNA残留，无RNase、核酸内、外切酶污染。
活性定义
在50℃、pH8.0 的条件下，1小时内使1nmol的 [3H] GMP 掺入酸不溶性沉淀物所需要的酶量定义为1个活性单位。
Thermostable T7 RNA polymerase is a genetically engineered T7 RNA polymerase. The enzyme highly specifically recognizes the T7 promoter sequence and can be highly transcribed in vitro at 50 ℃. In the process of transcription in vitro, it can improve the capping efficiency and eliminate the production of dsRNA by-products.
Product features
It has high specificity for T7 promoter and is used for the synthesis of RNA (including microRNA) in vitro.
Product usage
1) Synthesis of single stranded RNA;
2) Synthesis of highly specific RNA probes;
3) Synthetic siRNA precursor;
4) Making the precursor of RNA splicing reaction;
5) Cap analog was used as primer to make capped mRNA.
Usage suggestions
For efficient transcription of a specific region, it is suggested that the template DNA be pre cut into flat ends or 5 'protruding ends downstream of the region.
Spermidine in the buffer may combine with nucleic acid to form insoluble substance. It is suggested to add template DNA finally.
Storage temperature
-Long term storage at 20 ℃ or -80 ℃.
Quality assurance
After column purification for many times, only a clear and single target band can be seen by SDS-PAGE gel detection, with a purity of 95%. No E. coli DNA residue, RNase, endonuclease and exonuclease contamination can be detected by PCR.
Activity definition
Under the condition of 50 ℃ and ph8.0, the amount of enzyme required to add 1nmol of [3h] GMP into the acid insoluble precipitate within 1 hour is defined as 1 active unit.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 B2327171 |
分析证书 | T489384 |
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