Tris-乙酸缓冲液EDTA缓冲液作为运行缓冲液,用于人类乳头状瘤病毒DNA的琼脂糖凝胶电泳以及转录的RNA样品。TAE 电泳缓冲液是 DNA 琼脂糖凝胶电泳最常用的缓冲液,还用于非变性 RNA 琼脂糖凝胶电泳。双链 DNA 在 TAE 中往往比在其他缓冲液中移动得更快,但在长时电泳期间也会因缓冲液离子耗尽而无法泳动。在长时电泳期间进行缓冲液循环或缓冲液更换可弥补较低的缓冲能力。稀释浓缩的 TAE 缓冲液得到 1× TAE 缓冲液,含有 40mM Tris 乙酸盐和 1mM EDTA,pH 为 8.3。1× TAE 缓冲液既可用于琼脂糖凝胶中,也可用作电泳缓冲液。为达到最大分辨率,建议外加电压低于 5V/cm(单位电极间的距离)。
产品规格与参数:
货号
中文品名
规格
包装
T493081-01-1L
TRIS 醋酸盐-EDTA 缓冲液
10×;生物试剂,用于分子生物学;DNase和RNase;未检测到;粉末混合;适用于电泳
1L
T493081-02-10L
TRIS 醋酸盐-EDTA 缓冲液
适用于Northern 和 Southern印迹
10L
T493081-03-1L
TRIS 醋酸盐-EDTA 缓冲液
适用于电泳的生物试剂
1L
T493081-04-1L
TRIS 醋酸盐-EDTA 缓冲液
50×;在cGMP控制下设计和制造的试剂;适用于IVD应用
1L
T493081-05-1L
TRIS 醋酸盐-EDTA 缓冲液
10×;生物制剂,用于分子生物学,非无菌;0.2μm过滤
1L
T493081-05-4L
TRIS 醋酸盐-EDTA 缓冲液
10×;生物制剂,用于分子生物学;非无菌;0.2μm过滤
4L
T493081-06-1L
TRIS 醋酸盐-EDTA 缓冲液
10×;DNA酶和RNA酶;未检测到;生物试剂,适用于电泳
1L
T493081-06-5×1L
TRIS 醋酸盐-EDTA 缓冲液
10×;DNA酶和RNA酶;未检测到;生物试剂;适用于电泳
5×1L
Product application:
Tris acetic acid buffer EDTA buffer is used as the running buffer for agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples. Tae electrophoresis buffer is the most commonly used buffer for DNA agarose gel electrophoresis and is also used for non denaturing RNA agarose gel electrophoresis. Double stranded DNA often moves faster in TAE than in other buffers, but it will also be unable to swim due to the depletion of buffer ions during long-term electrophoresis. Buffer circulation or buffer replacement during long-term electrophoresis can compensate for lower buffer capacity. Dilute the concentrated TAE buffer solution to obtain 1 × TAE buffer, containing 40mM Tris acetate and 1mM EDTA, pH 8.3. one × Tae buffer can be used for both agarose gel and electrophoresis buffer. For maximum resolution, it is recommended that the applied voltage be less than 5V/cm (distance between unit electrodes).
Product specifications and parameters:
SKU
Name
Specification
Pack Size
T493081-01-1L
Tris Acetate-EDTA buffer
10×,BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis
1L
T493081-02-10L
Tris Acetate-EDTA buffer
for Northern and Southern blotting
10L
T493081-03-1L
Tris Acetate-EDTA buffer
suitable for electrophoresis
1L
T493081-04-1L
Tris Acetate-EDTA Buffer
50×,Reagent designed and manufactured under cGMP controls suitable for use in an IVD application
1L
T493081-05-1L
Tris Acetate-EDTA buffer
10×,BioReagent, for molecular biology, non-sterile; 0.2 μm filtered
1L
T493081-05-4L
Tris Acetate-EDTA buffer
10×,BioReagent, for molecular biology, non-sterile; 0.2 μm filtered
4L
T493081-06-1L
Tris Acetate-EDTA buffer
10×,DNase and RNase, none detected, BioReagent, suitable for electrophoresis
1L
T493081-06-5×1L
Tris Acetate-EDTA buffer
10×,DNase and RNase, none detected, BioReagent, suitable for electrophoresis