计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
TPC2-A1-N is a powerful and Ca 2+ -permeable agonist of two pore channel 2 (TPC2) , which plays its role by mimicking the physiological actions of NAADP. TPC2-A1-P reproducibly evokes significant Ca 2+ responses from TPC2 ( EC 50 =7.8 μM ), and the effect can be blocked by several TPC blockers. TPC2-A1-N can be used to probe different functions of TPC2 channels in intact cells
In Vitro
Two-pore channels (TPC1-3) are ancient members of the voltage-gated ion channel superfamily. TPCs are expressed throughout the endo-lysosomal system and regulates the trafficking of various cargoes. TPC2 can mediate different physiological and possibly pathophysiological effects depending on how it is activated. The ion selectivity of TPC2 is not fixed but rather agonist-dependent. TPC2 is a unique example of an ion channel that conducts different ions in response to different activating ligands. TPC2-A1-N (10 μM) reproducibly evokes Ca 2+ signals, and TPC2-A1-N response reachs its plateau faster than TPC2-A1-P. The EC 50 in full concentration-effect relationships for the plateau response is 7.8 μM for TPC2-A1-N in a cell line stably expressing TPC2 L11A/L12A . TPC2-A1-N (10 μM) evokes Ca 2+ influx through the TPC2 pore evokes Ca 2+ signals in cells expressing TPC2L11A/L12A but not TPC2 L11A/L12A/L265P . Additionally, the responses to TPC2-A1-N can be selectively blocked by the identified TPC2 blockers Tetrandrine , Raloxifene , and Fluphenazine by removal of extracellular Ca 2+. In endo-lysosomal patch-clamp experiments, TPC2-A1-N (30 μM) elicits currents using Na + as the major permeantion, in vacuolin-enlarged endo-lysosomes isolated from isolated from HEK293 cells transiently expressing human TPC2 (hTPC2) but not in cells expressing TPC1. In endo-lysosomal patch-clamp experiments, TPC2-A1-N (30 μM) induces larger currents in endo-lysosomes isolated from cells expressing a gain-of-function variant of TPC2 (TPC2 M484L ) compared to the wild-type isoform, and exhibits an EC 50 value of 0.6 μM for TPC2-A1-N. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
IC50& Target:EC50: 10.5 μM (Ca 2+ current response from TPC2)
规格
规格或纯度
≥99%
英文名称
TPC2-A1-N
生化机理
TPC2-A1-N是一种强效的双孔xa0通道xa02xa0（TPC2）钙2+渗透激动剂，它通过模拟xa0NAADP的生理作用来发挥作用。TPC2-A1-P可重复xa0引起TPC2的显著Ca 2+反应（EC 50 =7.8 μM），TPC2-A1-P是TPC2的生理作用xa0。
储存条件
-20°C储存
运输条件
超低温运输
纯度
≥99%
名称和识别符
分子类型
小分子
Smiles
O=C(NC1=CC=C(C(F)(F)F)C=C1)/C(C#N)=C(C2=CC(Cl)=CC(Cl)=C2)\\O
分子量
401.17
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 250 mg/mL (623.18 mM; Need ultrasonic)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥99%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 136186-07-7，分子式为 C17H9Cl2F3N2O2，分子量为 401.17 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 SSE:688179 保留所有权利。
危险品化学品经营许可证（带存储）