Uridine-5′-diphosphate glucose dehydrogenase, EC 1.1.1.22, derived from Pasteurella multocida. It catalyzes the formation of UDP-glucuronic acid, which is essential for hyaluronic acid synthesis. This product is recombinantly expressed in Escherichia coli.
Reaction:
Protocol for Use
Note: For scaled-up reactions, adjust the incubation time or enzyme amount based on actual conditions.
Add substrates to the reaction mixture. An appropriate buffer (e.g., Tris-HCl) may be added to adjust the pH to 8.0–10.0, followed by the addition of UDP-Glucose Dehydrogenase at a concentration of 0.1–0.5 mg/ml (adjust according to reaction requirements).
Mix thoroughly and incubate the sample at 37°C for 1–4 hours.
Terminate the reaction by incubating in an 80°C water bath for 10 minutes.
Detect by HPLC or TLC.
Precautions
For your safety and health, please wear lab coats and disposable gloves when handling. This product is for research use only!