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38、 产品介绍：
胆红素是胆色素的一种，它是人胆汁中的主要色素，是临床上判定黄疸的重要依据和肝功能的重要指标。
本试剂盒采用Harrison法或称哈里森法、哈里森试管法，检测原理是用氯化钡吸附尿中胆红素，滴加Fouchet试剂，使胆红素氧化成胆绿素而呈绿色反应，该试剂盒灵敏度较高，可达0.05mg/dl或0.9μmol/L，主要用于定性检测人、动物尿液中的胆红素。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）
1、取新鲜尿液4mL置于试管，加入氯化钡溶液约2mL，混匀，可产生白色沉淀。
2、3000g离心5min，弃上清液，留取沉淀。
3、向沉淀物中滴加Fouchet试剂数滴（1滴约50μL），若呈现绿色或蓝绿色者为阳性结果。
注意事项
1、尿液必须新鲜，取尿后立即检查，避免胆红素因为光线照射而分解。
2、尿液中如果含有水杨酸盐、阿司匹林类药物可呈假阳性。
3、不能加入过多的Fouchet试剂，以免生成黄色，导致假阴性。
4、试剂开封后请尽快使用，以防影响后续实验效果。
Bilirubin is a type of bile pigment and the main pigment in human bile. It serves as a crucial clinical indicator for diagnosing jaundice and assessing liver function.
This kit employs the Harrison method (also known as the Harrison tube test). The detection principle involves adsorbing bilirubin in urine using barium chloride, followed by adding Fouchet's reagent, which oxidizes bilirubin to biliverdin, producing a green color change. This kit offers high sensitivity, up to 0.05 mg/dL or 0.9 µmol/L, and is primarily used for the qualitative detection of bilirubin in human or animal urine. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.
Operating Steps (For Reference Only)
1. Place 4 mL of fresh urine in a test tube and add approximately 2 mL of Barium Chloride Solution. Mix well; a white precipitate will form.
2. Centrifuge at 3000 g for 5 minutes. Discard the supernatant and retain the precipitate.
3. Add a few drops (approximately 50 µL per drop) of Fouchet's Reagent to the precipitate. The appearance of a green or bluish-green color indicates a positive result.
Precautions
1. Urine must be fresh. Test immediately after collection to avoid degradation of bilirubin due to light exposure.
2. The presence of salicylates or aspirin in urine may cause false-positive results.
3. Avoid adding excessive Fouchet's Reagent, as this may produce a yellow color and lead to false-negative results.
4. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
| U1509474 | Component | 50T | Storage |
| U1509474A | 氯化钡溶液 | 100 mL | RT. |
| U1509474B | Fouchet试剂 | 10 mL | RT. Store in the dark. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333460 |
分析证书 | U1509474 |
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