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产品介绍
酮体是肝脏脂肪酸氧化分解的中间产物乙酰乙酸、β-羟基丁酸及丙酮三者统称。酮体中丙酮的生成量相当小，生成后即被吸收。乙酰乙酸和β-羟基丁酸则经血流进入肝外组织，在此被氧化，经柠檬酸循环提供更多能量给那些组织使用，例如骨、心肌和肾皮质。本试剂盒采用朗格 (Lange) 法进行检测，主要用于定性检测人、动物尿液中酮体含量。
本试剂盒检测原理如下：含酮体的尿液中加亚硝基铁氰化钠，在碱性溶液作用下生成紫色环，加入微量酸化液可防止假阳性。
产品组分
U1522043 | 组分名称 | 物理外观 | 50T | 储存 | 单次测试使用量 |
U1522043A | 试剂A：亚硝基铁氰化钠 | 粉末 | 10 g | RT. 避光 | 0.2 g |
U1522043B | 试剂B：冰乙酸（自备） | 液体 | 20 mL | RT. | 0.4 mL |
U1522043C | 试剂C：氨水（自备） | 液体 | 100 mL | RT. | 1.6 mL |
使用方法
1. 样本测定
（1）取新鲜尿液4mL于离心管中，加入亚硝基铁氰化钠0.2g，再加入冰乙酸0.4mL，反复振荡使其溶解。
（2）充分混匀，沿管壁慢慢加入氨水1.6mL，使之与尿液形成界面，静置后观察。
2. 结果判断
性 | 反应情况 | 相当含量（mg/L） | |
|
| 乙酰乙酸 | 丙酮 |
阴性 | 5min后无紫色环 | - | - |
可疑 | 只出现淡紫色环 | 50 | 200-400 |
+ | 逐渐出现淡紫色环 | 100 | 1000 |
2+ | 较快出现淡紫色环 | 200-400 | 2500-5000 |
3-4+ | 立即出现淡紫色环 | 1000-3000 | 8000-10000 |
注意事项
1. 当含有大量非晶形尿酸盐时，则产生黄色至褐色环。
2. 冰乙酸和氨水易挥发，建议在通风橱操作，并注意密闭保存。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Introduction
Ketone bodies are the collective term for three intermediate products of fatty acid oxidation in the liver: acetoacetic acid, β-hydroxybutyrate, and acetone. Acetone is produced in very small amounts and is rapidly absorbed upon formation. Acetoacetate and β-hydroxybutyrate enter extrahepatic tissues via the bloodstream, where they are oxidized through the citric acid cycle to provide additional energy to these tissues, such as bone, myocardium, and renal cortex. This test kit employs the Lange method for detection, primarily used for qualitative determination of ketone body levels in human and animal urine.
The principle of this test kit is as follows: when urine containing ketone bodies is mixed with sodium nitroprusside, a purple ring forms under alkaline conditions. A small amount of acidifying solution is added to prevent false positives.
Kit Contents
U1522043 | Component Name | Appearance | 50T | Storage | Quantity Per Test |
U1522043A | Reagent A: Sodium Nitroprusside | Powder | 10 g | RT. Store in the dark. | 0.2 g |
U1522043B | Reagent B: Glacial Acetic Acid (self-supplied) | Liquid | 20 mL | RT. | 0.4 mL |
U1522043C | Reagent C: Ammonia Solution (self-supplied) | Liquid | 100 mL | RT. | 1.6 mL |
Instructions for Use
1. Sample measurement
(1) Take 4 mL of fresh urine in a centrifuge tube, add 0.2 g of sodium nitroprusside, and then add 0.4 mL of glacial acetic acid. Shake repeatedly to dissolve.
(2) Mix thoroughly, slowly add 1.6 mL of ammonia solution along the wall of the tube to form an interface with the urine, and observe after standing.
2. Result judgment
| Qualitative | Reaction situation | Equivalent content (mg/L) | |
| Acetoacetic acid | Acetone | ||
| Negative | No purple ring after 5 minutes | - | - |
| Suspicious | Only a pale purple ring appears | 50 | 200-400 |
| + | Gradually appears a pale purple ring | 100 | 1000 |
| 2+ | Appears a pale purple ring quickly | 200-400 | 2500-5000 |
| 3-4+ | Immediately appears a pale purple ring | 1000-3000 | 8000-1000 |
Precautions
1. When there is a large amount of amorphous urate salts, a yellow to brown ring will be produced.
2. Glacial Acetic Acid and Ammonia Solution are prone to volatilization. It is recommended to operate in a fume hood and pay attention to keeping it sealed for storage.
3. For your safety and health, please wear a lab coat and disposable gloves during the operation.
U1522043 | 组分名称 | 物理外观 | 50T | 50T | 单次测试使用量 |
U1522043A | 试剂A：亚硝基铁氰化钠 | 粉末 | 10 g | RT. 避光 | 0.2 g |
U1522043B | 试剂B：冰乙酸（自备） | 液体 | 20 mL | RT. | 0.4 mL |
U1522043C | 试剂C：氨水（自备） | 液体 | 100 mL | RT. | 1.6 mL |
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