计算溶液所需的质量、体积或浓度。
BioReagent,分子生物学级,即用型 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿液由肾脏生成,是人体代谢终产物的主要排泄途径,其中含有白细胞、红细胞、上皮细胞等少量细胞成分,可作为 DNA 提取样本用于分子生物学检测。但尿液中尿素、尿酸等成分易降解核酸,且室温储存易滋生微生物,其分泌的核酸酶会造成 DNA 断裂、降解,直接影响 DNA 提取质量与产量。 本产品可高效稳定保护尿液中的 DNA,采集后的尿液直接与保存液混合即可,无需即时冻存,常温保存效果接近低温冻存,可满足任意场景采样及样本长途运输需求。
产品特点
使用方法
1. 按尿液:本产品 = 10:1体积比充分混合(如 100 mL 尿液加入 10 mL 保存液)。
2. 样本常温避光保存备用。
3. 采用常规 DNA 提取方法提取 DNA,产物可用于酶切、电泳、Southern 杂交、PCR 等分子生物学实验。
4. 如需长期低温保存,可将已混合保存液的尿液样本直接置于 - 80℃储存。
Urine is produced by the kidneys and serves as the primary excretion route for metabolic end products. It contains small amounts of cellular components such as leukocytes, erythrocytes, and epithelial cells, which can be used as samples for DNA extraction in molecular biology assays. However, components in urine such as urea and uric acid can easily degrade nucleic acids. Furthermore, storage at room temperature promotes microbial growth, and nucleases secreted by these microorganisms can cause DNA fragmentation and degradation, directly impacting the quality and yield of DNA extraction.
This product efficiently and stably protects DNA in urine. Collected urine can be mixed directly with the preservation solution without the need for immediate freezing. The preservation effect at room temperature is comparable to that of low-temperature freezing, making it suitable for sample collection in any scenario and for long-distance sample transportation.
Product Features
Easy to Use: Simply mix the urine sample with this product according to the specified ratio.
Long-Term Preservation: Stably preserves urine and its DNA for up to 6 months at 4–25°C, particularly suitable for field sampling.
High DNA Integrity: Suitable for total DNA extraction; the obtained DNA can be used in molecular biology experiments such as enzyme digestion, electrophoresis, and PCR.
Instructions
Mix the urine sample thoroughly with this product at a volume ratio of 10:1 (e.g., add 10 mL of preservation solution to 100 mL of urine).
Store the mixed sample protected from light at room temperature until use.
Extract DNA using a standard DNA extraction method. The extracted DNA can be used for molecular biology experiments such as enzyme digestion, electrophoresis, Southern blot hybridization, and PCR.
For long-term low-temperature storage, the urine sample mixed with the preservation solution can be stored directly at -80°C.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | U777329 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 Suitable for molecular biology 级别指南 →