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植物组织培养技术即植物无菌培养技术，又称离体培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官 (如根、茎、叶、花、茎尖、果实等)、组织 (如形成层、表皮、表层、髓部细胞、胚乳等) 或细胞 (大孢子、小孢子、体细胞等) 以及原生质体，在适宜的人工培养基及环境条件下，能够诱导出愈伤组织、不定芽、不定根，最后形成完整植株的技术。植物组织培养常用的培养基有 MS、NT、B5、N6、White、HDKW、Litvay、WPM 等。
SH 培养基即 Schenk&Hildebrandt 培养基，1972 年由 Schenk 和 Hildebrandt 设计，主要特点与 B5 培养基相似，是无机盐浓度较高的培养基。主要用于双子叶和单子叶植物悬浮细胞培养。与 MS、B5、N6、White、H 等培养基的基本成分相比，SH 培养基由磷酸氢二钠替代磷酸二氢钾，用磷酸二氢铵代替硝酸铵或硫酸铵，且肌醇含量较高，这是它的显著特点。由于配方中的离子浓度高，在配制、贮存和消毒等过程中，即使有些成分略有出入，也不会影响离子间的平衡。
SH 维生素溶液 (100×) 主要由肌醇、烟酸、盐酸吡哆醇、硫胺素等组成，是一种常用的植物组培用维生素混合液，作为相应的植物组培培养基基盐的添加剂使用。本品为 100× 母液，按照 1L 培养基中加入 10mL 的比例使用，不会造成培养基 pH 值的改变。本品未除菌，一般建议配制工作液后进行相关处理，防止微生物污染。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
1、使用方法：取 10 mL 本品加入 1 L 的培养基溶液中，混匀即可，调节至所需 pH 值，一般为 5.8±0.2。
2、根据需要，可于 121℃ 高压灭菌 15～20 min，用于植物组织培养。也可加入额外的碳源、凝胶、植物激素或生长调节剂等成分。
注意事项：
1、要根据不同的材料、不同的物种，选择合适的培养基，最好通过实验获得。
2、试剂开封后请尽快使用，以防影响后续实验效果。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Plant tissue‑culture, also known as axenic culture or in‑vitro culture, is a technique based on the theory of plant cell totipotency. Under suitable artificial medium and environmental conditions, isolated plant organs (such as roots, stems, leaves, flowers, shoot‑tips, fruits, etc.), tissues (such as cambium, epidermis, surface‑layer cells, pith cells, endosperm, etc.), cells (megaspores, microspores, somatic cells, etc.) and protoplasts can be induced to produce callus, adventitious buds and adventitious roots, and eventually develop into intact plantlets. Commonly‑used media for plant tissue‑culture include MS, NT, B5, N6, White, HDKW, Litvay, WPM and so on.
SH medium (Schenk & Hildebrandt medium) was designed by Schenk and Hildebrandt in 1972. Similar to B5 medium, it is a medium with a high concentration of inorganic salts and is mainly used for suspension‑cell culture of dicotyledonous and monocotyledonous plants. Compared with the basic components of MS, B5, N6, White, H and other media, SH medium uses disodium hydrogen phosphate instead of potassium dihydrogen phosphate, ammonium dihydrogen phosphate in place of ammonium nitrate or ammonium sulfate, and contains a relatively‑high‑content of myo‑inositol, which is its prominent feature. Thanks to its high‑ion‑concentration formula, slight deviations in some components during preparation, storage and sterilization will not affect the ionic equilibrium.
SH Vitamin Solution (100×) is a vitamin mixture widely used in plant tissue‑culture, mainly composed of myo‑inositol, nicotinic acid, pyridoxine hydrochloride, thiamine and other ingredients. It can be used as an additive for the basal salts of corresponding plant‑tissue‑culture media. This product is a 100 × stock solution. Add 10 mL of this solution to every 1 L of culture medium, and it will not change the pH value of the medium. This product is non‑sterile. It is recommended to perform sterilization treatment after preparing the working‑solution to prevent microbial contamination. This reagent is for research‑use‑only and shall not be applied for clinical‑diagnosis or other purposes.
Operating Procedure (For Reference Only):
1. Directions for use: Add 10 mL of this product into 1 L of medium solution and mix well. Adjust to the required pH value, generally 5.8±0.2.
2. If necessary, autoclave at 121 °C for 15‑20 min for plant‑tissue‑culture applications. Additional carbon‑sources, gelling agents, plant‑hormones or plant‑growth‑regulators may also be supplemented.
Precautions:
1. Select an appropriate culture‑medium according to your experimental materials and plant species. Optimal culture conditions are best determined experimentally.
2. Use the reagent as soon as possible after opening to avoid adverse impacts on subsequent experiments.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
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